In vitro cardiotoxicity assay system using MVP technology
| Project/Area Number |
25670126
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 心毒性 / 動きベクトル / QT延長作用 / 抗癌剤 / iPS細胞 / 心筋細胞 / 薬物応答 |
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| Outline of Final Research Achievements |
Cardiotoxicity of drugs is the main cause of drop out from the pipeline and recall from the market. Thus, the development of assay system, especially in vitro assay system in the early stage of drug development, has been strongly required from pharmaceutical companies. Here, we used multi-electrode array (MEA) system and motion vector prediction (MVP) system developed by the Sony coorporation to establish the system to simultaneously assay electrical activity and contractility in cardiomyocytes. We confirmed in human iPS-derived cardiomyocytes that drugs known to possess positive and negative inotropic effects could be reliably assayed. Recently, the cardiotoxicity of anti-cancer drugs has been strongly concerned. We successfully assayed cardiotoxicity of anticancer drugs.
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] ET-1 induces myofibrillar disarray and contractile vector in hypertrophic cardiomyopathy-iPS cell-derived cardiomyocytes2014
Author(s)
Tanaka A, Yuasa S, Mearini G, Egashira T, Seki T, Kodaira M, Kusumoto D, Kuroda Y, Okata S, Suzuki T, Arimura T, Makino S, Kimura K, Kimura A, Furukawa T, Carrier L, Nobe K, Fukuda K
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Journal Title
J Am Heart Assoc
Volume: 3
Issue: 6
Pages: 1-25
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] A multidisciplinary approach for evaluation of drug-induced QT prolongation using human induced pluripotent stem cell-derived cardiomyocytes2015
Author(s)
Kurokawa J, Okada J, Hayashi E, Ashihara T, Yoshinaga T, Sugiura S, Li M, Kanda Y, Sekino Y, Sawada K, Hisada T, Furukawa T
Organizer
59th Biophysical Society Annual Meeting
Place of Presentation
Baltimoreコンベンションセンター（米国）
Year and Date
2015-02-07
Related Report
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[Presentation] Genome-wide association study (GWAS) for a cardiac arrhythmia, atrial fibrillation2014
Author(s)
Furukawa T, Ebana Y, Nogami A, Nitta J, Kobayashi K, Mokura M, Takahashi Y, Hachiya H, Miyazaki S, Nakamura H, Hirao K, Isobe M, Ozaki K, Kubo M, Tanaka T
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
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[Presentation] Disease modeling using iPS cells2014
Author(s)
Furukawa T, Okata S, Yuasa S, Suzuki T, Makita N, Kurokawa J, Egashira T, Yamakawa H, Seki T, Aizawa T, Hashimoto H, Kuroda Y, Tanaka A, Yae K, Murata M, Aiba T, Shimizu W, Horie M, Kodama I, Ogawa S, Fukuda K
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
Invited
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[Presentation] Impact of SNP on IL6R (rs7514452) for age at onset of atrial fibrillation2014
Author(s)
Sato Y, Satoh A, Nitta J, Honda Y, Kuroda S, Sekigawa M, Kanoh M, Suzuki M, Inaba O, Muramatsu K, Yamato T, Matsumura Y, Asakawa K, Ebana Y, Furukawa T, Hirao K, Isobe M
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
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[Presentation] Impact of SNP on 1q24 (rsrs593479) in PRRX1 for the age at onset of atrial fibrillation2014
Author(s)
Sato Y, Satoh A, Nitta J, Honda Y, Kuroda S, Sekigawa M, Kanoh M, Suzuki M, Inaba O, Muramatsu K, Yamato T, Matsumura Y, Asakawa K, Ebana Y, Furukawa T, Hirao K, Isobe M
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
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[Presentation] Arrhythmogenicity of the superior vena cava and common single nucleotide polymorphisms in patients with paroxysmal atrial fibrillation2014
Author(s)
Takahashi Y, Ebana Y, Hayashi T, Miwa N, Masumura M, Goto K, Sakakibara A, Itoh J, Ohmi T, Ohno M, Katoh R, Nozato T, Satoh Y, Furukawa T, Hirao K, Isobe M
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
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[Presentation] The relationship between the single nucleotide polymorphism rs2634073 and arrhythmogenic superior vena cava in the patients with atrial fibrillation2014
Author(s)
Sato A, Honda Y, Sato Y, Kuroda S, Kanoh M, Sekigawa M, Suzuki M, Inaba O, Muramatsu K, Yamato T, Matsumura Y, Nitta J, Asakawa K, Ebana Y, Furukawa T
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
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[Presentation] Effect of SNP on 9q22 (rs6479562) on the progression from paroxysmal atrial fibrillation to persistent atrial fibrillation2014
Author(s)
Sekigawa M, Satoh A, Nitta J, Sato Y, Honda Y, Kuroda S, Kanoh M, Suzuki M, Inaba O, Muramatsu K, Yamato T, Matsumura Y, Asakawa K, Ebana Y, Furukawa T, Hirao K, Isobe M
Organizer
The 78th Annual Scientific Meeting of the Japanese Circulation Society
Place of Presentation
東京国際フォーラム、東京
Related Report
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[Presentation] Contractile behaviors of human-induced pluripotent stem cell-derived cardiomyocyte monolayers evaluated with an image-based analysis using motion vector prediction technique: A comparison with extracellular electrophysiology2013
Author(s)
Hayakawa T, Kunihiro T, Ando T, Unno H, Kobayashi S, Matsui E, Yada H, Kanda Y, Kurokawa J, Furukawa T
Organizer
13th annual meeting of Safety Pharmacology Society. Rotterdam
Place of Presentation
Rotterdam, Netherland
Related Report
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