Challenge to the accurate detection of minimal drug resistant cells
Project/Area Number |
25670184
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Hokkaido University |
Principal Investigator |
OHBA Yusuke 北海道大学, 医学(系)研究科(研究院), 教授 (30333503)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | バイオイメージング / 腫瘍 / 薬剤耐性 / フローサイトメーター / FRET / CML / 蛍光寿命 |
Outline of Final Research Achievements |
In Philadelphia chromosome-positive leukemia, growth and survival of leukemia cells solely depend on constitutive tyrosine kinase activity of new fusion gene product BCR-ABL formed by chromosome reciprocal translocation between chromosomes 9 and 22. Therefore, the treatment for this disease has been radically improved by the introduction of the specific tyrosine kinase inhibitor of BCR-ABL. However, it is desired to develop new technologies that enable the detection of a minimal number of drug-resistant cells because substantial patients experience drug resistance regardless of the presence or absence of past and current treatment. We have previously developed a novel technique to detect relatively small number of drug resistant cells. Here, we challenged to centuplicate the detection ability by the improvement of the FRET biosensor, the detection method and the analytical method. As a result, we obtained 162-fold improvement throughout this study.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] CRK adaptor protein induces epithelial-mesenchymal transition and metastasis of bladder cancer cells via HGF/c-Met feedback loop.2015
Author(s)
Matsumoto R, Tsuda M, Wang L, Maishi N, Abe t, Kimura T, Tanino M, Nishihara H, Hida K, Ohba Y, Shinohara N, Nonomura K, Tanaka S.
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Journal Title
Cancer Sci
Volume: in press
Issue: 6
Pages: 709-17
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Inhibition of multidrug transporter in tumor endothelial cells enhances antiangiogenic effects of low-dose metronomic paclitaxel2015
Author(s)
1.Akiyama K., Maishi N., Ohga N., Hida Y., Ohba Y., Alam Mohammad Towfik, Kawamoto T., Ohmura H., Yamada K., Torii C., Shindoh M. and *Hida K.
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Journal Title
Am J Pathol
Volume: 185(2)
Issue: 2
Pages: 572-580
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Agonist-promoted ubiquitination differentially regulates receptor trafficking of endothelin type A and type B receptors.2014
Author(s)
Terada K., Horinouchi T., Fujioka Y., Higashi T., Nepal P., Horiguchi M., Sarita K., Hatate C., Hoshi A., Harada T., Mai Y., Ohba Y. and Miwa S.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 51
Pages: 35283-35295
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Beneficial innate signaling interference for anti-bacterial responses by a TLR-mediated enhancement of the MKP-IRF3 axis2013
Author(s)
Negishi H, Matsuki K, Endo N, Sarashina H. Miki S, Matsuda A, Fukazawa K, Taguchi-Atarashi N, Ikushima H, Yanai H, Nishio J, Honda K, Fujioka Y, Ohba Y, Noda T, Taniguchi S, Nishida E, Zhang Y, Chi H, Flavell RA, Taniguchi T
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Journal Title
Proc Nat1 Acad Sci U S A
Volume: 49
Issue: 49
Pages: 19884-19889
DOI
Related Report
Peer Reviewed
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