| Project/Area Number |
25670268
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
KITAJIMA Isao 富山大学, 大学院医学薬学研究部(医学), 教授 (50214797)
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Hideki 富山大学, 大学病院, 助教 (50401865)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 感染症 / 微生物 / 敗血症 / 遺伝子検査 / 転写因子 / 1分子計測 / 一分子計測 |
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| Outline of Final Research Achievements |
The earliest possible identification of pathogenic microorganisms is critical for selecting appropriate antimicrobial therapy. We developed a nested-PCR-based assay for detecting Mycoplasma and other bacteria in amniotic fluid samples using eukaryotic, thermostable DNA polymerase. Next, a "melting temperature (Tm) mapping method" was developed for identifying a broad range of bacteria. Employing primer sets for a 16S ribosomal gene, more than 100 bacterial species can be identified. Nuclear transcription factor-kappa B (NF-kB) activation plays a key role in the regulation of immune responses to inflammation. We developed an experimental apparatus for detection of NF-kB activation based on fluorescence correlation spectroscopy (FCS), which permits analysis of DNA-protein binding in the liquid phase. FCS was performed using the single-molecule fluorescence detention system. We have integrated Tm mapping and the FCS based NF-kB assay and are conducting clinical trials in sepsis patients.
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