Bile acid profiles in cholangiocarcinoma developing in printing workers for the application to early diagnosis

• Project/Area Number: 25670319
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health
• Research Institution: Kyoto Prefectural University
• Principal Investigator: MINAMIYAMA Yukiko 京都府立大学, 生命環境科学研究科(系), 教授 (00362989)
• Co-Investigator(Kenkyū-buntansha): TAKEMURA Shigekazu 大阪市立大学, 大学院医学研究科, 講師 (00322363) ; OKA Mayuko 京都府立大学, 大学院生命環境科学研究科, 准教授 (40347498)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 酸化ストレス / 二次胆汁酸 / 炎症 / 胆汁 / 酸化還元動態 / 変異原性 / 胆汁酸 / チオール動態

Outline of Final Research Achievements

Cholangiocarcinoma that epidemically occurred among young adult workers in the printing company (PC) becomes a social problem in Japan. It is speculated that the causes some organic solvents. This study was aimed to explore the specific tumor promoter for the PC practitioners in bile obtained in special outpatient clinic and surgical treatment of Osaka City University Hospital. Bile samples were analyzed mutagenicity, redox status (lipid peroxide, thiol analysis), and bile acid composition in cholangiocaricinoma of thePC workers and the others, choledocholithiasis, pancreatic cancer, and liver cancer. Comparing the printing industry workers and other cholangiocarcinoma, the ratio of oxidized glutathione and many secondary bile acids were increased. These results suggested to be caused by potentially elevated the long-acting bile duct inflammation, the change of intestinal flora. We will continue the research for a new carcinogenic promoter on the PC workers.

Research Products

• [Journal Article] 3.Outbreak of cholangiocarcinoma developing in printing company workers : a new type of occupational cancer. (2014)
  • Author(s): Kubo S, Takemura S, Sakata C, Urata Y, Nozawa A, Nishioka T, Kinoshita M, Hamano G, Tanaka S, Sugawara Y, Nakanuma Y, Endo G.
  • Journal Title: Nihon Shokakibyo Gakkai Zasshi Volume: 111 Pages: 500-509
  • Peer Reviewed
• [Journal Article] Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan. (2014)
  • Author(s): Sato Y, Kubo S, Takemura S, Sugawara Y, Tanaka S, Fujikawa M, Arimoto A, Harada K, Sasaki M, Nakanuma Y.
  • Journal Title: Int J Clin Exp Pathol. Volume: 15 Pages: 4745-4754
  • Peer Reviewed
• [Journal Article] Changes in laboratory test results and diagnostic imaging presentation before the detection of occupational cholangiocarcinoma. (2014)
  • Author(s): Kubo S, Takemura S, Sakata C, Urata Y, Nishioka T, Nozawa A, Kinoshita M, Hamano G, Nakanuma Y, Endo G.
  • Journal Title: J Occup Health. Volume: 56 Pages: 317-322
  • NAID: 130004447660
  • Peer Reviewed
• [Journal Article] Characteristics of printing company workers newly diagnosed with occupational cholangiocarcinoma. (2014)
  • Author(s): Kubo S, Kinoshita M, Takemura S, Tanaka S, Shinkawa H, Nishioka T, Hamano G, Ito T, Abue M, Aoki M, Nakagawa K, Unno M, Hijioka S, Fujiyoshi T, Shimizu Y, Mizuguchi T, Shirabe K, Nishie A, Oda Y, Takenaka K, Kobarai T, Hisano T, Saiura A, Numao H, Toda M, Kuwae Y, Nakanuma Y, Endo G.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 21 Issue: 11 Pages: 809-817
  • DOI: 10.1002/jhbp.137
  • Peer Reviewed
• [Journal Article] Case series of 17 patients with cholangiocarcinoma among young adult workers of a printing company in Japan. (2014)
  • Author(s): Kubo S, Nakanuma Y, Takemura S, Sakata C, Urata Y, Nozawa A, Nishioka T, Kinoshita M, Hamano G, Terajima H, Tachiyama G, Matsumura Y, Yamada T, Tanaka H, Nakamori S, Arimoto A, Kawada N, Fujikawa M, Fujishima H, Sugawara Y, Tanaka S, Toyokawa H, Kuwae Y, Ohsawa M, Uehara S, Sato KK, Hayashi T, Endo G.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 21 Issue: 7 Pages: 479-488
  • DOI: 10.1002/jhbp.86
  • Peer Reviewed / Open Access
• [Presentation] 印刷労働者関連胆管癌と他の胆管癌の臨床病理学的比較 (2014)
  • Author(s): 濱野 玄弥、 竹村 茂一、坂田 親治、浦田 順久、西岡 孝芳、野沢 彰紀、木下 正彦、伊藤 得路、久保 正二
  • Organizer: 第69回日本消化器病学会総会
  • Place of Presentation: 郡山
  • Year and Date: 2014-07-18