Method for assessment of stress coping using microRNA

• Project/Area Number: 25670352
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine(including psychosomatic medicine)
• Research Institution: The University of Tokushima
• Principal Investigator: ROKUTAN Kazuhito 徳島大学, ヘルスバイオサイエンス研究部, 教授 (10230898)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ストレス / ストレスコーピング / マイクロRNA / バイオマーカー / 末梢血

Outline of Final Research Achievements

It has been suggested that microRNA plays a crucial role in stress coping reactions. In this project, we succeeded to identify chronic psychological stress-responsive microRNAs (miR-16, miR-20b, miR-26b, miR-29a, miR126, miR-144, miR-144*) (PLoS One, 2013). We have also identify an anxiety and/or depression-associated miR-144 in healthy medical staffs of private hospitals. In addition, we examined differentially expressed microRNAs in peripheral blood from patients with chronic fatigue syndrome, and we revealed 6 microRNASs differentially expressed in those patients. Using the Target scan software, we found that the differentially expressed microRNAs significantly augmented inflammatory reaction and suppressed the protein synthesis pathway. Our results suggest that distinct microRNAs in peripheral blood may be useful for assessment of stress responses in healthy subjects and for diagnosis of pathological fatigue.

Research Products

• [Journal Article] Transformer 2β and miR-204 regulate apoptosis through competitive binding to 3’UTR of BCL2 mRNA. (2015)
  • Author(s): Kuwano Y, Nishida K, Kajita K, Satake Y, Akaike Y, Fujita K, Kano S, Masuda K, Rokutan K.
  • Journal Title: Cell Death Differ. Volume: 22 (5) Issue: 5 Pages: 815-825
  • DOI: 10.1038/cdd.2014.176
  • Peer Reviewed / Open Access
• [Journal Article] HuR regulates alternative splicing of the TRA2 β gene in human colon cancer cells under oxidative stress. (2014)
  • Author(s): Akaike Y, Masuda K, Kuwano Y, Nishida K, Kajita K, Kurokawa K, Satake Y, Shoda K, Imoto I, Rokutan K.
  • Journal Title: Mol Cell Biol Volume: 34 Issue: 15 Pages: 2857-73
  • DOI: 10.1128/mcb.00333-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oxidative stress-induced truncated serine/arginine-rich splicing factor 3 regulates interleukin-8 production in human colon cancer cells. (2014)
  • Author(s): Kano S, Nishida K, Kurebe H, Nishiyama C, Kita K, Akaike Y, Kajita K, Kurokawa K, Masuda K, Kuwano Y, Tanahashi T, Rokutan K.
  • Journal Title: Am J Physiol Cell Physiol. Volume: 306 (3) Issue: 3 Pages: 250-262
  • DOI: 10.1152/ajpcell.00091.2013
  • Peer Reviewed / Open Access
• [Journal Article] Downregulation of serine/arginine-rich splicing factor 3 induces G1 cell cycle arrest and apoptosis in colon cancer cells. (2014)
  • Author(s): Kurokawa K, Akaike Y, Masuda K, Kuwano Y, Nishida K, Yamagishi N, Kajita K, Tanahashi T, Rokutan K.
  • Journal Title: Oncogene. Volume: 33 Issue: 11 Pages: 1407-17
  • DOI: 10.1038/onc.2013.86
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Truncated serine/arginine-rich splicing factor 3 accelerates cell growth through up-regulating c-Jun expression. (2013)
  • Author(s): Kano S, Nishida K, Nishiyama C, Akaike Y, Kajita K, Kurokawa K, Masuda K, Kuwano Y, Tanahashi T, Rokutan K.
  • Journal Title: J Med Invest. Volume: 60 Pages: 228-235
  • NAID: 130004822687
  • Peer Reviewed
• [Journal Article] Chronic academic stress increases a group of microRNAs in peripheral blood (2013)
  • Author(s): Honda M, Kuwano Y, Katsuura-Kamano S, Kamezaki Y, Fujita K, Akaike Y, Kano S, Nishida K, Masuda K, Rokutan K
  • Journal Title: PLoS One Volume: 8(10) Issue: 10 Pages: e75960-e75960
  • DOI: 10.1371/journal.pone.0075960
  • Peer Reviewed
• [Journal Article] NF90 in posttranscriptional gene regulation and microRNA biogenesis. (2013)
  • Author(s): Masuda K, Kuwano Y, Nishida K, Rokutan K, Imoto I.
  • Journal Title: Int J Mol Sci. Volume: 14(8) Issue: 8 Pages: 17111-21
  • DOI: 10.3390/ijms140817111
  • Peer Reviewed
• [Journal Article] Chronic inhibition of tumor cell-derived VEGF enhances the malignant phenotype of colorectal cancer cells. (2013)
  • Author(s): Yamagishi N, Teshima-Kondo S, Masuda K, Nishida K, Kuwano Y, Dang DT, Dang LH, Nikawa T, Rokutan K.
  • Journal Title: BMC Cancer Volume: 13 Issue: 1 Pages: 229-229
  • DOI: 10.1186/1471-2407-13-229
  • Peer Reviewed
• [Journal Article] Ets1 and heat shock factor 1 regulate transcription of the Transformer 2β gene in human colon cancer cells. (2013)
  • Author(s): Kajita K, Kuwano Y, Kitamura N, Satake Y, Nishida K, Kurokawa K, Akaike Y, Honda M, Masuda K, Rokutan K.
  • Journal Title: J Gastroenterol. Volume: 48(11) Issue: 11 Pages: 1222-33
  • DOI: 10.1007/s00535-012-0745-2
  • Peer Reviewed
• [Journal Article] Molecular features of triple negative breast cancer cells by genome-wide gene expression profiling analysis. (2013)
  • Author(s): Komatsu M, Yoshimaru T, Matsuo T, Kiyotani K, Miyoshi Y, Tanahashi T, Rokutan K, Yamaguchi R, Saito A, Imoto S, Miyano S, Nakamura Y, Sasa M, Shimada M, Katagiri T.
  • Journal Title: Int J Oncol. Volume: 42(2) Issue: 2 Pages: 478-506
  • DOI: 10.3892/ijo.2012.1744
  • Peer Reviewed
• [Journal Article] Associations between chronotype and salivary endocrinological stress markers (2013)
  • Author(s): Toda M, Kawai T, Takeo K, Rokutan K, Morimoto K
  • Journal Title: Endocr Res Volume: 38 Issue: 1 Pages: 1-7
  • DOI: 10.3109/07435800.2012.683225
  • Peer Reviewed
• [Presentation] ストレスバイオマーカーの探索と創薬への応用 (2014)
  • Author(s): 六反一仁
  • Organizer: 第30回日本DDS学会学術集会 ランチョンセミナー
  • Place of Presentation: 慶応義塾大学薬学部芝共立キャンパス（東京都・港区）
  • Year and Date: 2014-07-30 – 2014-07-31
  • Invited
• [Presentation] ストレス研究からみた病的疲労の評価 シンポジウム「ストレス・睡眠・疲労」 (2014)
  • Author(s): 六反一仁
  • Organizer: 第10回日本疲労学会総会・学術集会
  • Place of Presentation: 大阪コングレコンベンションセンター（大阪府・大阪市）
  • Year and Date: 2014-05-31 – 2014-06-01
  • Invited