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The treatment to reduce the rejection in xenotransplantation - Pretransplant replacement of donor liver grafts with recipient liver cells.

Research Project

Project/Area Number 25670554
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionKyoto University

Principal Investigator

UEMOTO Shinji  京都大学, 医学研究科, 教授 (40252449)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords肝移植 / 異種移植 / 骨髄細胞移植 / Kupffer細胞 / 骨髄細胞 / 拒絶軽減 / 細胞置換
Outline of Final Research Achievements

We investigated whether preoperative transplant of recipient-derived bone marrow cells (BMCs) to the donor attenuated the rejection after liver transplantation in the rat acute rejection model. Rats in the BMC group experienced prolonged survival. Serological and histopathological analyses revealed that the rejection of the liver graft was attenuated in the BMC group. X-gal staining and immunohistostaining of the liver graft revealed that transplanted BMCs were engrafted in the sinusoidal space and differentiated into Kupffer cells. Preoperative transplantation of recipient BMCs to LT donors replaced donor Kupffer cells and attenuated post-LT rejection, indicating that this strategy may increase the success of LT. We are planning further investigations with xenotransplantation models. We established the hamster/rat liver xenotransplantation model, which is similar to pig/human xenotransplantation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (11 results)

  • [Journal Article] Pretransplant replacement of donor liver grafts with recipient Kupffer cells attenuates liver graft rejection in rats.2015

    • Author(s)
      Endo K, Hori T, Jobara K, Hata T, Tsuruyama T, Uemoto S
    • Journal Title

      Journal of Gastroenterology and Hepatology

      Volume: 30 Issue: 5 Pages: 944-951

    • DOI

      10.1111/jgh.12872

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 血液型不適合移植(肝、腎)の現況と課題への対応 Rituximabを用いた血液型不適合肝移植成績2015

    • Author(s)
      八木眞太郎・上本伸二 他
    • Organizer
      日本移植学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドミノ肝移植の現状と今後の展望 当院におけるドミノ肝移植の現況と展望2015

    • Author(s)
      濱口雄平・上本伸二 他
    • Organizer
      日本移植学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体肝移植ドナーの安全性向上に向けた取り組み2015

    • Author(s)
      海道利実・上本伸二 他
    • Organizer
      日本移植学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 生体肝移植における術前サルコペニアの意義2015

    • Author(s)
      濱口雄平・上本伸二 他
    • Organizer
      日本移植学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 当院脳死肝移植における予後因子の検討2015

    • Author(s)
      奥村晋也・上本伸二 他
    • Organizer
      日本移植学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肝移植と門脈圧亢進症 肝移植手術における門脈圧制御の重要性2015

    • Author(s)
      八木眞太郎・上本伸二 他
    • Organizer
      日本門脈圧亢進症学会
    • Place of Presentation
      横浜
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラット15%過小グラフト肝移植、95%肝切除における門脈下大静脈シャントによる門脈圧制御の肝再生促進効果2015

    • Author(s)
      政野裕紀・上本伸二 他
    • Organizer
      肝胆膵外科学会総会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肝移植ドナーに対するレシピエント由来骨髄細胞移植による肝グラフト細胞置換および拒絶軽減の試み2014

    • Author(s)
      遠藤耕介、堀智英、城原幹太、畑俊行、 鶴山竜昭、上本伸二
    • Organizer
      日本外科学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2014-04-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Preoperative bone marrow cell transplantation to the donor reduced the rejection after the liver transplantation in the rat acute rejection model2013

    • Author(s)
      Kosuke Endo, Tomohide Hori, Kanta Jobara, Toshiyuki Hata, Shinji Uemoto
    • Organizer
      Digestive Disease Week 2013
    • Place of Presentation
      Orlando, United States
    • Related Report
      2013 Research-status Report
  • [Presentation] ドナーに対する肝移植術前骨髄移植による肝構成細胞の置換と拒絶軽減の可能性についての検討2013

    • Author(s)
      遠藤耕介、堀智英、城原幹太、畑俊行、上本伸二
    • Organizer
      第25回日本肝胆膵外科学会・学術集会
    • Place of Presentation
      宇都宮市
    • Related Report
      2013 Research-status Report
  • [Presentation] Impact of recursive engraftment of host bone-marrow cells into allogeneic liver grafts on subsequent rat liver transplantation characterized by strong rejection2013

    • Author(s)
      Kosuke Endo, Tomohide Hori, Kanta Jobara, Toshiyuki Hata, Tatsuaki Tsuruyama, Shinji Uemoto
    • Organizer
      13th congress of the asian society of transplantation
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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