| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
|---|
| Outline of Final Research Achievements |
This study revealed that adenine addition enhances butanol production in Clostridium saccharoperbutylacetonicum cultivated at 37°C. When strain N1-4 cultivated at 37°C with the addition of adenine, the intracellular ATP level was improved. In addition, although strain N1-4 cultivated at 37°C without the addition of adenine sporulated since 6 hours, spore formation was inhibited until 24 hours with the addition of adenine at even 37°C. We isolated heat resistant mutant RIFR91 using rifampicin as an inhibitor and analyzed characteristic of the strain.
|
