Structure and function of paralytic neurotoxins and proteases from terrestrial mammals
| Project/Area Number |
25702047
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Kita Masaki 筑波大学, 数理物質系, 准教授 (30335012)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Keywords | 天然物化学 / 神経毒 / 単離と構造 / 生物活性 / プロテアーゼ |
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| Outline of Final Research Achievements |
In this study, I have conducted neurotoxins and proteases from venomous mammals, including shrews and solenodons. The short-tailed shrew (Blarina brevicauda) produce a lethal protein venom in saliva. After removing proteins by ultracentrifugation, the smaller molecular weight fraction showed significant paralytic activity against mealworms and mice (icv injection). Based on this activity, two peptide neurotoxins with molecular weights of 5.1 and 5.7 kDa were purified, and their primary amino acid sequences were established. As for Cuban solenodon (Solenodon cubanus), ecological survey was conducted in a national park in Cuba. Several individuals were successfully taken alive, and their saliva was collected. The partial amino acid sequences and activity of one of the major proteases in solenodon saliva was determined.
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Report
(4 results)
Research Products
(63 results)
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[Presentation] Study on the Target Proteins of Antitumor Macrolide Aplyronine A2014
Author(s)
Yuichiro Hirayama, Kozo Yoneda, Kota Yamagishi, Kota Tsuchiya, Takumi Chinen, Takeo Usui, Eriko Sumiya, Motonari Uesugi, Tomohiro Suzuki, Hirokazu Kawagishi, Masaki Kita, and Hideo Kigoshi
Organizer
3rd Asian Chemical Biology Conference (ACBC3)
Place of Presentation
シンガポール国立大学（シンガポール）
Year and Date
2014-12-14 – 2014-12-18
Related Report
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[Presentation] アクチン脱重合活性を有する海洋産マクロリド、ミカロライドBの全合成2014
Author(s)
岡大峻, 臼井明裕, 石塚智也, 茂木雄三, 渡邉秀和, 角田真樹, 松木麻衣子, 木村智之, 栗林里実, 末井敬太, 上田祥子, 仙石哲也, 早川一郎, 末永聖武, 北将樹, 木越英夫
Organizer
第56回天然有機化合物討論会
Place of Presentation
高知県立県民文化ホール（高知県）
Year and Date
2014-10-15 – 2014-10-17
Related Report
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