| Budget Amount *help |
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
|
|---|
| Outline of Final Research Achievements |
I developed a probe to visualize single-molecule dynamics of telomere repeat-containing RNA (TERRA) in living cells to reveal mechanism of TERRA function in telomere maintenance. I performed single molecule imaging of TERRA and telomere in living U2OS cells using the present probe. Analysis of spatial distribution of TERRA and telomere revealed that TERRA intensively localize in a region 1-2 μm apart from a telomere. Next I performed simultaneous single molecule observation of TERRA, telomere, and hnRNPA1 which is a telomere-related protein. The results of this observation showed that TERRA and hnRNPA1 frequently colocalize to each other in the telomere-surrounding region. On the basis of these results, I propose a novel mechanistic model of TERRA, in which TERRA in the telomere-surrounding region traps hnRNPA1 and control the function of hnRNPA1 to maintain telomere length.
|
