| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Plants regulate guard cell differentiation in response to environmental cues including CO2 levels. Some reports have shown that elevated CO2 concentrations suppress stomatal development, but other studies could not reproduce the effect. To clarify this issue, quantitative imaging analysis of stomatal pattern formation was conducted with Arabidopsis cotyledons grown under ambient and elevated CO2 conditions. Our microscopic image analysis revealed that elevated CO2 did not affect stomatal density/index but perturbed the uniform distribution of stomata, with excess satellite stomata and meristemoids. We then used an overexpression line of a gene for a DNA replication licensing factor, CDC6, which was reported to positively regulate satellite meristemoid production. The overexpressor showed hypersensitivity to elevated CO2-induced stomatal distribution changes, suggesting that elevated CO2 positively regulates CDC6-mediated satellite stomata production, at least in Arabidopsis cotyledons.
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