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Molecular mechanisms of silica-accumulating soil bacteria and their role in the silicon cycle

Research Project

Project/Area Number 25712009
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

Ikeda Takeshi  広島大学, 先端物質科学研究科, 助教 (10505754)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2013: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Keywords細菌 / 胞子 / ケイ素
Outline of Final Research Achievements

We previously reported that Bacillus cereus and its close relatives accumulate silicon (Si) in and around a spore coat layer. Si is taken up from the environment as soluble silicate (Si[OH]4), which is then polymerized and accumulated as insoluble silica (SiO2). The spore coat is a proteinaceous shell composed of more than 50 proteins. Silica accumulation in and around the spore coat strongly suggests that spore coat proteins play an important role in silica formation. In this study, we found that one of the spore coat proteins, CotB1, mediates the accumulation of silica.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] The C-terminal zwitterionic sequence of CotB1 is essential for biosilicification of the Bacillus cereus spore coat2016

    • Author(s)
      Kei Motomura, Takeshi Ikeda, Satoshi Matsuyama, Mohamed A. A. Abdelhamid, Tatsuya Tanaka, Takenori Ishida, Ryuichi Hirota, Akio Kuroda
    • Journal Title

      Journal of Bacteriology

      Volume: 198 Issue: 2 Pages: 276-282

    • DOI

      10.1128/jb.00447-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag2014

    • Author(s)
      M.A.A. Abdelhamid, K. Motomura, T. Ikeda, T. Ishida, R. Hirota, and A. Kuroda
    • Journal Title

      Appl. Microbiol. Biotechnol

      Volume: 98 Issue: 12 Pages: 5677-5684

    • DOI

      10.1007/s00253-014-5754-z

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bacillus属細菌における新規ケイ酸トランスポーターの探索2015

    • Author(s)
      田中 達也, 本村 圭, 池田 丈, 廣田 隆一, 黒田 章夫
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新規シリカ結合タグの開発と鹿児島県産シリカ含有鉱物「シラス」を担体とした低コストアフィニティー精製法への応用2015

    • Author(s)
      池田 丈, Mohamed A. A. Abdelhamid, 本村 圭, 廣田 隆一, 黒田 章夫
    • Organizer
      城山観光ホテル(鹿児島県鹿児島市)
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Silica-based affinity purification using silica-binding tags2015

    • Author(s)
      Mohamed A. A. Abdelhamid, Kei Motomura, Takeshi Ikeda, Takenori Ishida, Ryuichi Hirota, Akio Kuroda
    • Organizer
      Cambridge Healthtech Institute's 14th Annual PepTalk: The Protein Science Week (PepTalk 2015)
    • Place of Presentation
      Town and Cuntry Resort & Convention Center, San Diego, CA, USA
    • Year and Date
      2015-01-19 – 2015-01-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Bacillus cereusのシリカ蓄積機構における胞子タンパク質CotB1の役割2014

    • Author(s)
      本村 圭, 池田 丈, Mohamed A. A. Abdelhamid, 廣田 隆一, 黒田 章夫
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Bacillus cereusのシリカ蓄積機構に関与する胞子タンパク質の同定2013

    • Author(s)
      本村 圭, 池田 丈, 小西 浩司, Mohamed A. A. Abdelhamid, 廣田 隆一, 黒田 章夫
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Affinity purification of recombinant proteins using a novel silica-binding2013

    • Author(s)
      Mohamed A. A. Abdelhamid, Kei Motomura, Takeshi Ikeda, Ryuichi Hirota, Akio Kuroda
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 精製方法、および、当該精製方法に用いられる酸化ケイ素結合タグ2014

    • Inventor(s)
      黒田 章夫, 池田 丈, 舟橋 久景
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-215789
    • Filing Date
      2014-10-22
    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] ペプチドおよびその利用2013

    • Inventor(s)
      池田 丈, 本村 圭, 黒田 章夫
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-173945
    • Filing Date
      2013-08-23
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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