Study on physicochemical process of radiation damage for DNA adsorbed on self-assembled monolayer

• Project/Area Number: 25740020
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: National Institute of Advanced Industrial Science and Technology (2014-2015); Japan Atomic Energy Agency (2013)
• Principal Investigator: Narita Ayumi 国立研究開発法人産業技術総合研究所, 省エネルギー研究部門, 産総研特別研究員 (50633898)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: DNA / 自己組織化単分子膜 / 放射光軟X線 / X線光電子分光 / 吸収端近傍X線吸収微細構造 / 放射線影響 / 吸収端近傍X線吸収微細構造法 / DNA損傷過程 / 生体分子固定化 / 酸化物表面 / 軟X線 / X線吸収微細構造法 / X線光電子分光法 / 放射光

Outline of Final Research Achievements

A novel two-layer sample composed of a deoxyribonucleic acid (DNA) film and self-assembledmonolayer (SAM) was prepared on an inorganic surface. A SAM was formed on a sapphire surface, then oligonucleotide (OGN) molecules were adsorbed on the MPTS-SAM. The thicknesses and chemical states of the layers were determined. The sample was irradiated with synchrotron soft X-rays. The chemical state of the OGN molecules before and after irradiation was examined by Near-edge X-ray fine structure (NEXAFS). The peak shape of the N K-edge NEXAFS spectra of the MPTS-OGN layers clearly changed following irradiation.The MPTS-OGN layer formed on the sapphire surface. The chemical states and the structure of the interface were elucidated using synchrotron soft X-rays. The OGN molecules adsorbed on the MPTS films decomposed upon exposure to soft X-ray irradiation.

Research Products

• [Journal Article] Use of a DNA film on a self-assembled monolayer for investigating the physical process of DNA damage induced by core electron ionization (2016)
  • Author(s): Ayumi Narita, Kentaro Fujii, Yuji Baba, Iwao Shimoyama
  • Journal Title: International Journal of Radiation Biology Volume: - Issue: 11 Pages: 733-738
  • DOI: 10.1080/09553002.2016.1179812
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bond Cleavage of Adenosine 5’-triphosphate Induced by Monochromatic Soft X-rays (2014)
  • Author(s): K. Fujii, A. Narita, A. Yokoya
  • Journal Title: Journal of Physics Confefence Series Volume: 502 Pages: 12034-12034
  • DOI: 10.1088/1742-6596/502/1/012034
  • Peer Reviewed / Open Access
• [Journal Article] DNA damage by soft X-ray exposure at oxygen K-edge (2013)
  • Author(s): Y. Sugaya, A. Narita, K. Fujii, and A. Yokoya
  • Journal Title: Journal of Physics:ConferenceSeries Volume: 502 Pages: 12040-12040
  • DOI: 10.1088/1742-6596/502/1/012040
  • Peer Reviewed / Open Access
• [Presentation] Novel technique to investigate physical process of DNA damage induction by core electron ionization of constituent atoms in chromatin structure (2015)
  • Author(s): Ayumi Narita, Kentaro Fujii, Akinari Yokoya, Yuji Baba, Iwao Shimoyama
  • Organizer: 8th Auger Symposium
  • Place of Presentation: JAEA Kyoto, Kidsu, Kyoto, Japan
  • Year and Date: 2015-05-20
  • Int'l Joint Research
• [Presentation] 自己組織化単分子膜を介した無機表面へのオリゴヌクレオチドの固定化 (2015)
  • Author(s): 成田 あゆみ、藤井 健太郎、横谷 明徳、馬場 祐治、下山 巖
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 有機自己組織化膜をアンカーとした東名酸化物表面へのオリゴヌクレオチドの固定化 (2015)
  • Author(s): 成田あゆみ、藤井 健太郎、横谷 明徳、馬場 祐治、下山 巖
  • Organizer: 第3回物構研サイエンスフェスタ
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-03-17 – 2015-03-18
• [Presentation] Immobilization of oligonucleotide on oxide surface through organic layers as an anchor (2014)
  • Author(s): Ayumi Narita, Kentaro Fujii, Akinari Yokoya, Yuji Baba, Iwao Shimoyama
  • Organizer: 7th International Symposium on Surface Science (ISSS-7)
  • Place of Presentation: くにびきメッセ
  • Year and Date: 2014-11-02 – 2014-11-06
• [Presentation] 酸化物表面に作製した有機自己組織化膜への オリゴヌクレオチド固定化 (2014)
  • Author(s): 成田あゆみ、藤井健太郎、横谷明徳、馬場祐治、下山巖
  • Organizer: 第2回物構研サイエンスフェスタ
  • Place of Presentation: 茨城県つくば市 つくば国際会議場
• [Presentation] Immobilization of alkyl-chain molecules on inorganic surface through functional group (2013)
  • Author(s): Ayumi Narita, Yuji Baba, Tetsuhiro Sekiguchi, Iwao Shimoyama, Norie Hirao, Tsuyoshi Yaita
  • Organizer: Light and Particle Beams in Materials Science 2013
  • Place of Presentation: 茨城県つくば市 つくば国際会議場