Multiple analysis of intracellular responases in intractable disease related to dopaminergic neuron vulnerability with the use of Parkinson's Disease specific iPS cells
| Project/Area Number |
25830055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Hoshi University (2014)
Keio University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | iPS 細胞 / パーキンソン病 / メタボローム / ドパミン神経 / メタボローム解析 / 疾患特異的iPS 細胞 |
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| Outline of Final Research Achievements |
In the present study, iPSCs were generated from two Parkinson’s patients and two control subjects. All of the clones differentiated into neurons included tyrosine hydroxylase-positive neurons. Under the present condition, I found differences in gene expression between control and patients. Among those, the expression level of PGC-1α, which plays a key role in mitochondrial biogenesis and energy metabolism, in Parkinson’s specific-iPS cell (PD-iPSCs)-derived dopaminergic (DA) neurons was significantly lower than that in control. Using CE-MS-system, I found the change in the expression of several metabolites in glycolysis and glutathione metabolism pathways in DA neuron-derived from PD-iPSCs. Interestingly, the expression of S-adenosylmethionone, which can lead to methylation of DNA, was significantly increased in PD-iPSCs-derived DA neurons. These findings suggest that metabolic abnormality in DA neurons could lead to neuronal dysfunction in PD.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Changes in circadian rhythm for mRNA expression of melatonin 1A and 1B receptors in the hypothalamus under a neuropathic pain-like state.2014
Author(s)
Odo M, Koh K, Takada T, Yamashita A, Narita M, Kuzumaki N, Ikegami D, Sakai H, Iseki M, Inada E, Narita M.
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Journal Title
Synapse
Volume: 68
Issue: 4
Pages: 153-158
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epigenetic transcriptional activation of monocyte chemotactic protein 3 contributes to long-lasting neuropathic pain.2013
Author(s)
Imai S, Ikegami D, Yamashita A, Shimizu T, Narita M, Niikura K, Furuya M, Kobayashi Y, Miyashita K, Okutsu D, Kato A, Nakamura A, Araki A, Omi K, Nakamura M, James Okano H, Okano H, Ando T, Takeshima H, Ushijima T, Kuzumaki N, Suzuki T, Narita M.
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Journal Title
Brain
Volume: 136
Issue: 3
Pages: 828-843
DOI
Related Report
Peer Reviewed
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[Journal Article] Kappa-Opioids inhibit tumor angiogenesis by suppressing VEGF signaling.2013
Author(s)
Yamamizu K, Furuta S, Hamada Y, Yamashita A, Kuzumaki N, Narita M, Doi K, Katayama S, Nagase H, Yamashita JK, Narita M.
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 3213-3213
DOI
Related Report
Peer Reviewed / Open Access
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