| Project/Area Number |
25830070
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | National Research Institute for Child Health and Development |
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Principal Investigator |
MATSUDA Go 独立行政法人国立成育医療研究センター, 母児感染研究部, 研究員 (60392130)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 感染症 / ウイルス / 癌 / 人工ヌクレアーゼ / 遺伝子治療 / 応用動物 / トランスレーショナルリサーチ |
|---|
| Outline of Final Research Achievements |
Epstein-Barr virus (EBV) is associated with various neoplastic diseases. EBNA1 can be a target of anti-viral therapy because EBNA1 is expressed in all EBV associated diseases and is an essential factor for maintaining viral genome and persistence in infected cells. The aim of this study is to remove EBV genome from infected cells by targeting EBNA1 regions in EBV genome using artificial nuclease techniques.
In this research, we showed that CRISPR/Cas9 system could cleave EBNA1 regions effectively in HeLa cells. But the efficiency of viral gene editing in EBV infected cells was not enough. Furthermore we succeeded the preparation of model mice of EBV infectious diseases. We will try new therapy using gene editing in the model mice as soon as the efficiency is increased.
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