| Project/Area Number |
25830094
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
HATTORI Naoko 独立行政法人国立がん研究センター, 研究所, 研究員 (30611090)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | エピジェネティクス / ヒストン修飾 / ES細胞 / 幹細胞 / クロモドメイン |
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| Outline of Final Research Achievements |
In this study, to identify histone reader proteins involved in the maintenance of pluripotency of normal and cancer stem cells, we analyzed the function of chromodomain protein, Cdyl2. Mouse embryonic stem cells (ESC) with Cdyl2 reduction by knock down system and with increase expression of exogenous Cdyl2 showed that the differentiation ability of ESC was perturbed by the change of Cdyl2 expression level. ChIP assay revealed that Cdyl2 was enriched at the promoter regions of differentiation-associated genes. Furthermore, immunoprecipitation-western blotting showed that Cdyl2 co-localized with EZH2, and recognized H3K27me3. These findings demonstrate that Cdyl2 is involved in the maintenance of pluripotency in ESC, and suggest that Cdyl2 represses development-associated genes to keep an undifferentiated state.
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