Elucidation of chromosome condensation mechanism using premature chromosome condensation system and phosphoproteomics

• Project/Area Number: 25840073
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: TAKATA Hideaki 大阪大学, 工学(系)研究科(研究院), 助教 (20455207)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: クロマチン / 染色体 / リン酸化プロテオミクス / Chromosome condensation / Phospoproteomics / Phosphorylation / Chromatin / Chromosome / Chromatin condensation / Proteomics / Cell cycle

Outline of Final Research Achievements

The objective of this study is identification of phosphorylated proteins from condensed chromatin to elucidate the mechanism of chromatin condensation during mitosis. We identified some unknown phosphorylated proteins during mitosis in addition to known phosphorylated proteins. Furthermore, we found the tendency that proteins which are closely related to chromosome structure get phosphorylation during chromatin condensation. It suggests that protein phosphorylation has key functions to trigger chromosome condensation.

Research Products

• [Journal Article] Chromosome Interior Observation by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) Using Ionic Liquid Technique. (2014)
  • Author(s): Hamano T., Dwiranti A., Kaneyoshi K., Fukuda S., Kometani R., Nakao M., Takata H., Uchiyama S., Ohmido N., and Fukui K.
  • Journal Title: Microsc. Microanal. Volume: 20 Issue: 5 Pages: 1340-1347
  • DOI: 10.1017/s143192761401280x
  • Peer Reviewed
• [Journal Article] The Effect of Magnesium Ions on Chromosome Structure as Observed by Helium Ion Microscopy. (2014)
  • Author(s): Dwiranti, A., Hamano, T., Takata, H., Nagano, S., Guo, H., Ohnishi, K, Wako, T., Uchiyama, S., and Fukui, K.
  • Journal Title: Microsc. Microanal. Volume: 20 Issue: 1 Pages: 184-188
  • DOI: 10.1017/s1431927613013792
  • Peer Reviewed
• [Journal Article] クロマチンの凝縮が生み出す放射線耐性 (2014)
  • Author(s): 髙田英昭, 前島一博
  • Journal Title: Isotope News Volume: 719 Pages: 2-6
• [Journal Article] Chromatin compaction protects genomic DNA from radiation damage. (2013)
  • Author(s): Takata, H., Hanafusa, T., Mori, T., Shimura, M., Iida,Y., Ishikawa, K., Yoshikawa, K., Yoshikawa, Y., Maeshima, K.
  • Journal Title: PLOS ONE Volume: 8 Issue: 10 Pages: e75622-e75622
  • DOI: 10.1371/journal.pone.0075622
  • Peer Reviewed / Open Access
• [Journal Article] ヒトゲノムDNAの不規則で柔軟な収納原理 (2013)
  • Author(s): 前島一博, 城地保昌, 西野吉則, 髙田英昭, 鎌田福美, 日原さえら
  • Journal Title: 生物物理 Volume: 53 Pages: 4-10
• [Presentation] ゲノムクロマチンの凝縮が生み出す新たな放射線耐性 (2014)
  • Author(s): 髙田英昭
  • Organizer: 染色体学会第65回年会
  • Place of Presentation: 倉敷市芸分館
  • Year and Date: 2014-10-24
• [Presentation] The biological significance of chromatin condensation with irregular folding of nucleosome fiber (2014)
  • Author(s): Hideaki Takata
  • Organizer: U.K.-Japan Chromosome Workshop
  • Place of Presentation: Research complex at Harwell (U.K.)
• [Remarks] 大阪大学福井研究室
  • URL: http://www.bio.eng.osaka-u.ac.jp/cl/top.php
• [Remarks] DNAを有害な放射線から守る新しい仕組み
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2013/10/20131010_1