Identification of novel double fertilization regulators locating on the female gamete surface
| Project/Area Number |
25840113
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Chiba University |
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Principal Investigator |
Igawa Tomoko 千葉大学, 大学院園芸学研究科, 助教 (00360488)
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| Research Collaborator |
MORI Toshiyuki
YAMADA Lixy
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 卵細胞 / プロテオーム / 膜タンパク質 / 被子植物 / 生殖 / 配偶体 / 花粉管 / 重複受精 / 配偶子 |
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| Outline of Final Research Achievements |
Arabidopsis transgenic plant (EC-PM-GFP), where the egg cell plasma membrane is specifically labeled with GFP, was produced. The protein extract derived from collected pistils were subjected to immnoprecipitation using anti-GFP antibody. As a result, specific proteins such as GFP were successfully identified only in the proteins from transgenic pistils, but not in those of wild type pistils. It was indicated that egg cell proteins within the pistil cell population were successfully isolated by our method. This method has a great advantage of avoiding laborious work to handle the egg cell.
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Report
(5 results)
Research Products
(14 results)
