The elucidation and the application for breeding of red skin coloration mechanism of bud sport of European pear

Research Project

Project/Area Number	25850016
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Horticultural science
Research Institution	Yamagata University
Principal Investigator	IKEDA Kazuo 山形大学, 農学部, 准教授 (80555269)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	セイヨウナシ / 果皮色 / アントシアニン
Outline of Final Research Achievements	The pear fruits usually show green or yellow skin, but some cultivars show red skin caused by bud mutation.In this study, we found that these red skin color was caused by accumulation of the anthocyanin and it required sunlight. Also, solid type and brush type was present in red skin cultivars, but it was suggested that each red skin cultivar has inherited accumulation pattern except for coloration type. Gene expression analysis and DNA blot analysis of genes correlated with anthocyanin accumulation showed large size deletion or insertion did not exist in coding region of those genes, suggesting exist of SNPs or deletion/insertion in non-coding region.