| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We aimed at understanding the effect of lignin on enzymatic saccharification of cellulosic biomass comprehensively using not isolated lignins but some real biomass samples. Steam-exploded eucalyptus was mainly used for our experiments. First, we successfully developed a method to study cellulase adsorption behavior by calculating the amount of protein adsorbed on the substrate based on elemental analysis data. The amount of cellulase adsorbed reached maximum at the beginning stage, and was gradually decreased probably due to detachment from cellulose as the cellulose was hydrolyzed. Cellulase remained in the solution even at lower enzyme loadings, and the amount of adsorbed cellulase was increased with the increase of lignin content of the substrate as saccharification proceeds. It was suggested that the lignin suffered from alkaline pretreatment adsorbs more cellulase than that with other pretreatments.
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