Gene network analysis of placental function regulators and a seed gene, ezrin
| Project/Area Number |
25860396
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胎児発育不全 / 血液胎盤関門 / 遺伝子ネットワーク / 胎児成長不全 / エズリン / ヒポタウリン / 遺伝子ネットワーク解析 / トランスポーター / 胎盤 |
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| Outline of Final Research Achievements |
Placental function plays important roles in the fetal growth. Ezrin is a regulatory gene for plasma membrane proteins in the placenta, and deficiency of ezrin induces fetal growth retardation (FGR). This study aimed to clarify a gene network between ezrin and the other genes responsible for FGR by means of informatics and experiments in order to understand the molecular mechanism of the ezrin-mediated FGR. Network analysis showed no direct relationship between ezrin and the known FGR-related genes, indicating that ezrin-mediated FGR includes the novel molecular mechanism. We further analyzed protein interaction between Ezrin and a hypotaurine transporter, SLC6A13 in the placenta, since Ezrin knockout fetal mice showed deficiency of hypotaurine. Ezrin and SLC6A13 are co-localized on the plasma membrane in the labyrinth zone of mouse placenta. These results indicate that a novel interaction between Ezrin and SLC6A13 is important for fetal growth.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Fetal growth retardation and lack of hypotaurine in ezrin knockout mice2014
Author(s)
Nishimura T, Higuchi K, Sai Y, Sugita Y, Yoshida Y, Tomi M, Wada M, Wakayama T, Tamura A, Tsukita S, Soga T, Nakashima E.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] System A amino acid transporter SNAT2 shows subtype-specific affinity for betaine and hyperosmotic inducibility in placental trophoblasts2014
Author(s)
Nishimura T, Yagi R, Usuda M, Oda K, Yamazaki M, Suda S, Takahashi Y, Okazaki F, Sai Y, Higuchi, K, Maruyama T, Tomi M, Nakashima E
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Journal Title
Biochim Biophys Acta
Volume: VOL. 1838
Pages: 1306-12
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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