Improvement of quantitative assay of allergens in processed foods containing inhibiting factors.
| Project/Area Number |
25860474
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene and public health
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| Research Institution | Osaka City Institute of Public Health and Environmental Sciences |
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Principal Investigator |
Murakami Taro 大阪市立環境科学研究所, 調査研究課, 研究主任 (70393254)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | アレルギー物質 / ELISA / 特定原材料 / ポリビニルピロリドン / 小麦グルテン / プロアントシアニジン / 小麦グリアジン / DMAC / Polyvinylpyrrolidone / MALDI-TOFMS / 阻害因子 / 落花生 / ＥＬＩＳＡ / ポリフェノール |
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| Outline of Final Research Achievements |
Enzyme-linked immunosorbent assay (ELISA) is commonly used to detect trace amounts of food allergens; however, it is plausible that ELISA might be affected by co-existence of matrices from various food ingredients. We found that extraction solutions containing proanthocyanidins (PACs) inhibited allergens determination with ELISA, especially for gluten quantification. The methods was improved by adding polyvinylpyrrolidone (PVP), a PACs-binding agent, to the extraction buffer, and it was found that for the detection of gluten in cocoa powder, addition of 1% PVP K15 was most effective to prevent the inhibition. This new extraction method can be applied as a more precise and reliable tool for determining food allergens in processed foods.
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Report
(5 results)
Research Products
(10 results)
