| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Enzyme-linked immunosorbent assay (ELISA) is commonly used to detect trace amounts of food allergens; however, it is plausible that ELISA might be affected by co-existence of matrices from various food ingredients. We found that extraction solutions containing proanthocyanidins (PACs) inhibited allergens determination with ELISA, especially for gluten quantification. The methods was improved by adding polyvinylpyrrolidone (PVP), a PACs-binding agent, to the extraction buffer, and it was found that for the detection of gluten in cocoa powder, addition of 1% PVP K15 was most effective to prevent the inhibition. This new extraction method can be applied as a more precise and reliable tool for determining food allergens in processed foods.
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