Generating tissue-engineered small intestine (TESI) from cultured intestinal stem cells.

Research Project

Project/Area Number	25861202
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Digestive surgery
Research Institution	Nagasaki University
Principal Investigator	TORASHIMA Yasuhiro 長崎大学, 医歯薬学総合研究科(医学系), 講師 (40534467)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	TESI / 人工小腸 / 小腸上皮幹細胞 / 小腸間質系幹細胞 / 培養細胞 / 小腸幹細胞 / Lgr5 cell / 消化管再生
Outline of Final Research Achievements	The procedure for generating TESI from mature intestinal cells was established in a mouse model. Then, TESI was successfully generated from Lgr5 stem cells with further differentiation into intestinal epithelium after 4 weeks of in vivo implantation.