| Project/Area Number |
25861708
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 表皮角化細胞 / 真皮線維芽細胞 / 培養皮膚 / 血管内皮前駆細胞 / 無血清生体外培養増幅法 / 複合型培養皮膚 / 非選別末梢血単核球 / 無血清生体外培養細胞 |
|---|
| Outline of Final Research Achievements |
Keratinocytes and fibroblasts were respectively co-cultured with endothelial progenitor cells obtained by a serum-free quality and quantity control culture (QQc). We compared the number of cells of keratinocytes and fibroblasts in co-cultured with the number of their in mono-cultured after 48hours. As a result, for keratinocytes, the increase in cell number of approximately 29% (n = 1) was found in co-culture. For fibroblasts, the increase in cell number of approximately 11% (n = 3) was found in co-culture. It is reasonable to suppose that QQc cells have a proliferative effect on each cells.
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