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Generation of the odontogenic epithelial cell by Thymosin beta 4 gene introduction

Research Project

Project/Area Number 25861747
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

FUJIWARA Hiroaki  九州大学, 歯学研究科(研究院), 助教 (50634200)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords歯の再生 / シグナル伝達 / Thymosin beta 4 / Runx2 / シグナル伝達経路
Outline of Final Research Achievements

Odontogenic epithelial cells which product enamel, and odontogenic mesenchymal cells which product dentin or pulp are essential for tooth regeneration. However, the reports of the generation of odontogenic epithelial cells are hardly seen. Therefore, the aim of this study is to generate the odontogenic epithelium cells by using gene transfection of Thymosin beta 4 (TMSB4X) and to elucidate its mechanism. As a result, after TMSB4X transfection into human keratinocytes, the cell were transformed into odontogenic epithelium-like cells which had ability to form the enamel-like calcified material and express enamel-related protein. In addition, Smad and PI3K-Akt signaling pathways were suggested as this signaling pathway.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Thymosin beta 4 is associated with RUNX2 expression via the Smad and Akt signaling pathways in mouse dental epithelial cells2015

    • Author(s)
      Someya H., Fujiwara H.†, Nagata K., Wada H., Hasegawa K., Mikami Y., Jinno A., Sakai H., Koyano K. and Kiyoshima T. (†: Corresponding author)
    • Journal Title

      Int. J. Mol. Med.

      Volume: 35 Issue: 5 Pages: 1169-1178

    • DOI

      10.3892/ijmm.2015.2118

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Induction of dental epithelial cell differentiation marker gene expression in non-odontogenic human keratinocytes by transfection with thymosin beta 4.2013

    • Author(s)
      Kiyoshima T, Fujiwara H, Nagata K, Wada H, Ookuma YF, Shiotsuka M, Kihara M, Hasegawa K, Someya H, Sakai H
    • Journal Title

      Stem Cell Res

      Volume: 12 Pages: 309-322

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 非歯原性上皮細胞へのThymosin beta 4 遺伝子導入による歯原性上皮細胞への誘導2014

    • Author(s)
      藤原弘明、清島保、永田健吾、和田裕子、坂井英隆
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      ANAクラウンプラザホテル広島(広島)
    • Year and Date
      2014-04-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Thymosin beta 4遺伝子導入による歯原性上皮細胞の作製

    • Author(s)
      藤原弘明、清島保、永田健吾、和田裕子、木原槇子、長谷川佳那、染矢祐孝、坂井英隆
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス歯肉上皮由来角化細胞へのThymosin beta 4遺伝子導入による歯原性上皮細胞誘導

    • Author(s)
      染矢祐孝、清島保、永田健吾、和田裕子、藤原弘明、木原槇子、長谷川佳那、古谷野潔、坂井英隆
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
    • Related Report
      2013 Research-status Report
  • [Remarks] Thymosin beta 4 遺伝子導入による歯原性上皮細胞作成の 可能性

    • URL

      http://www.kyushu-u.ac.jp/pressrelease/2013/2013_12_03.pdf

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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