Evaluation of prospectively isolated dental pulp stem cells in vivo and application to jawbone necrosis therapy

• Project/Area Number: 25861895
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Keio University
• Principal Investigator: YASUI TAKAZUMI 慶應義塾大学, 医学部, 共同研究員 (80348771)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 歯髄幹細胞 / 細胞表面マーカー / フローサイトメトリー / 骨再生 / 再生医療 / 歯髄 / 幹細胞 / 骨形成 / 顎骨壊死

Outline of Final Research Achievements

Human dental pulp stem cells (hDPSCs) isolated by plastic adherence, frequently contain contaminating hematopoietic cells and mature cells, which has limited our ability to investigate their basic biology and regenerative properties. In the present study, we prospectively isolated a pure population of hDPSCs from human dental pulp by flow cytometry. Prospectively isolated, dental pulp-derived LNGFR Low+/THY-1 High+ cells represent a highly enriched population of clonogenic cells; notably, the isolated cells exhibited high proliferation and multi-lineage differentiation potential in vitro. The cells also promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFR Low+/THY-1 High+ dental pulp-derived cells provide an excellent source of material for bone regenerative strategies.

Research Products

• [Journal Article] Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration. (2015)
  • Author(s): Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD, Morikawa S, Onizawa K, Kawana H, Akazawa C, Suzuki N, Nakagawa T, Okano H, Matsuzaki Y.
  • Journal Title: journal of Dental Research Volume: 95 Issue: 2 Pages: 206-214
  • DOI: 10.1177/0022034515610748
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 予期的に分離した高純度歯髄幹細胞による骨形成能の評価 (2013)
  • Author(s): 安居孝純, 鬼澤勝弘，軽部健史, 森川 暁, 河奈裕正, 中川種昭
  • Organizer: 第57回日本口腔外科学会総会
  • Place of Presentation: マリンメッセ福岡（福岡県）