| Project/Area Number |
25861896
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 神経堤細胞 / 毛包 / 再生医学 / 硬組織再生 / 歯胚再生 / 細胞分化 / 分子生物学 / 口腔生化学 / 再生歯学 / 細胞分化・組織形成 / スフェロイド培養 |
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| Outline of Final Research Achievements |
Neural crest(NC) cells are maintained in an undifferentiated state as NC-derived cells (NCDCs) throughout the life of the animal. We utilized P0-Cre/floxed-EGFP mice (P0 mice), in which NCDCs remain labeled with green fluorescent protein (GFP) even after birth. We observed proliferation and differentiation of NCDCs harvested from whisker pad in adult P0 mice.
NCDCs harvested from whisker pad proliferate stably in certain conditions, and the expression of neural crest-related genes was observed.Our findings demonstrate that NCDCs have the potential to differentiate into osteoblasts, adipocytes, and neuronal cells. Expression of odontoblast marker genes was confirmed at the contact aerials of the respective cells when co-cultured with ameloblasts and NCDCs.Hard tissue regenerative capacity of NCDCs also have been evaluated in vivo.
NCDCs from the whisker pad , which are easily accessible in adult, can be cultured and may be a useful cell source for regeneration of the hard tissue.
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