Confirm new mechainism of oral malodor

• Project/Area Number: 25862069
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Fukuoka Dental College
• Principal Investigator: MASUO YOSUKE 福岡歯科大学, 歯学部, 助教 (40631693)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 口臭産生のメカニズム / β-ガラクトシダーゼ / 口臭 / 口腔内細菌 / 舌苔 / β-galactosidase

Outline of Final Research Achievements

Volatile sulfur compounds (VSCs) are produced by proteolysis of periodontopathic bacteria. The proteolysis of glycoproteins depends on the initial removal of the carbohydrate side chains, and β-galactosidase in saliva is considered to play a significant role in that reaction. The aim of the present study was to investigate the localization of β-galactosidase and to evaluate the relationship between this enzyme activity and parameters related to oral malodor.β-galactosidase activity was detected in the pellet lysate and whole saliva, but not in the saliva supernatant: the activity level was the same in the pellet lysate and whole saliva. There was a positive correlation between β-galactosidase activity and total protein in the saliva pellet, although total protein was not related to malodor strength.Our results indicate that β-galactosidase is located on the cell surface of oral bacteria and plays an important role in producing the malodor underlying physiological halitosis.

Research Products

• [Journal Article] Effect of S-PRG eluate on oral biofilm and oral malodor. (2014)
  • Author(s): Suzuki N,Yoneda M,Haruna K,Masuo Y,Nishihara K,Yamada K,Fujimoto A,Hirofuji T.
  • Journal Title: Arch Oral Biol. Volume: 59 Pages: 407-413
  • Peer Reviewed
• [Journal Article] Lactobacillus salivarius WB21-containing tablets for the treatment of oral malodor: a double-blind, randomized, placebo-controlled crossover trial. (2014)
  • Author(s): Suzuki N, Yoneda M, Tanabe K, Fujimoto A, Iha K, Seno K, Yamada K, Iwamoto T, Masuo Y, Hirofuji T
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology Volume: 117 Issue: 4 Pages: 462-470
  • DOI: 10.1016/j.oooo.2013.12.400
  • Peer Reviewed / Open Access
• [Presentation] Improvement of oral malodor using Lactobacillus salivarius WB21-containig tablets. (2013)
  • Author(s): Suzuki N,Yoneda M,Tanabe K,Fujimoto A,Iha K,Seno K,Iwamoto T,Yamada K,Masuo Y,Haruna K,and Hirofuji T.
  • Organizer: WCPD
  • Place of Presentation: Budapest
• [Presentation] ヒノキチオール配合ジェルを用いた口腔ケアの口臭改善効果 (2013)
  • Author(s): 岩元知之,鈴木奈央,米田雅裕,山田和彦,桝尾陽介,藤本暁江,瀬野恵衣,春名一人,安忠大,入江丹佳子,西原哲世,仲西宏介,廣藤卓雄
  • Organizer: 総合歯科協議会
  • Place of Presentation: 東京
• [Presentation] S-PRG溶出液が口腔バイオフィルムと口臭に及ぼす阻害作用 (2013)
  • Author(s): 春名一人,鈴木奈央,米田雅裕,山田潤一,桝尾陽介,西原哲世,仲西宏介,山田和彦,岩元知之,藤本暁江,伊波幸作,瀬野恵衣,安忠大,入江丹佳子,廣藤卓雄
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 秋田市