Non-equiliblium energy conversion efficiency measurement of molecular motor kinesin

• Project/Area Number: 25870173
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics; Biophysics
• Research Institution: Kyushu University (2014-2015); The University of Tokyo (2013)
• Principal Investigator: Takayuki Ariga 九州大学, 理学(系)研究科(研究院), 特任准教授 (30452262)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 1分子計測・操作 / 生体物質の物理 / 非平衡物理学 / エネルギー変換 / 生体分子モーター / １分子計測・操作

Outline of Final Research Achievements

A large variety of bio-molecular machines are working in cells. Especially, kinesin is walking on the microtubule rail to carry various cargos. We investigated the mechano-chemical efficiencies on the kinesin molecular motor by using a novel non-equilibrium equation through both experimental measurement and simulation approaches. The results showed kinesin's non-equilibrium behaviors and revealed that they do not produce so much futile non-equilibrium dissipation.

Research Products

• [Journal Article] Photoclickable Dendritic Molecular Glue : Noncovalent-to-Covalent Photochemical Transformation of Protein Hybrids (2013)
  • Author(s): Noriyuki Uchida, Kou Okuro, Yamato Niitani, Xiao Ling, Takayuki Arifja, Michio Tomishige, Takuzo Aida
  • Journal Title: Journal of the American Chemical Society Volume: 135 Issue: 12 Pages: 4684-4687
  • DOI: 10.1021/ja401059w
  • Peer Reviewed
• [Journal Article] Single molecule FRET observation of kinesin-1's head-tail interaction on microtubule (2013)
  • Author(s): T. Aoki, M. Tomishige, T. Ariga
  • Journal Title: BIOPHYSICS Volume: 9 Issue: 0 Pages: 149-159
  • DOI: 10.2142/biophysics.9.149
  • NAID: 130003383784
  • ISSN: 1349-2942
  • Peer Reviewed
• [Presentation] Non-equiliblium energetics of molecular motor kinesin (2016)
  • Author(s): 有賀隆行
  • Organizer: ゆらぎと構造の協奏第3回領域研究会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2016-06-17
  • Invited
• [Presentation] Dual-feedback microrheology in cytoskeletal networks (2016)
  • Author(s): N. Honda, K. Nishizawa, T. Ariga, D. Mizuno
  • Organizer: APS March Meeting 2016
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2016-03-17
  • Int'l Joint Research
• [Presentation] 多重フィードバックによる細胞骨格のマイクロレオロジー計測 (2015)
  • Author(s): 本田菜月, 西澤賢治, 有賀隆行, 水野大介
  • Organizer: 第5回ソフトマター研究会
  • Place of Presentation: 宮城県仙台
  • Year and Date: 2015-12-18
• [Presentation] Non-equiliblium energy measurement of molecular motor kinesin (2015)
  • Author(s): T. Ariga, M. Tomishige, D. Mizuno
  • Organizer: International Symposium on Fluctuation and Structure out of Equilibrium 2015
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2015-08-20
  • Int'l Joint Research
• [Presentation] 「Dual-feedback microrheology in cytoskeletal networks (2015)
  • Author(s): N. Honda, K. Nishizawa, T. Ariga, T. Yanagishima, D. Mizuno
  • Organizer: International Symposium on Fluctuation and Structure out of Equilibrium 2015
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2015-08-20
  • Int'l Joint Research
• [Presentation] 生体分子モーターキネシンの非平衡エネルギー計測 (2015)
  • Author(s): 有賀隆行、富重道雄、水野大介
  • Organizer: ゆらぎと構造の協奏第2回公開シンポジウム
  • Place of Presentation: 東京大学 本郷キャンパス
  • Year and Date: 2015-05-01
• [Presentation] 分子モーターキネシンの非平衡エネルギー計測 (2015)
  • Author(s): 有賀隆行、富重道雄、水野大介
  • Organizer: ゆらぎと構造の協奏第2回公開シンポジウム
  • Place of Presentation: 東京都文京区
  • Year and Date: 2015-04-30
• [Presentation] フィードバックマイクロレオロジーによるソフトマターのゆらぎと応答の観測 (2015)
  • Author(s): 本田菜月, 西澤賢治, 有賀隆行, 柳島大輝, 水野大介
  • Organizer: 日本物理学会第70回年次大会
  • Place of Presentation: 早稲田大学
  • Year and Date: 2015-03-21 – 2015-03-24
• [Presentation] Fluctuation and response of soft materials measured by dual-feedback microrheology (2015)
  • Author(s): N. Honda, K. Nishizawa, T. Ariga, T. Yanagishima, D. Mizuno
  • Organizer: International Conference "Physics of Structural and Dynamical Hierarchy in Soft Matter"
  • Place of Presentation: 東京大学生産技術研究所
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 生体分子モーターキネシンの非平衡エネルギー計測 (2014)
  • Author(s): 有賀隆行、富重道雄、水野大介
  • Organizer: 領域横断研究会「細胞力学と細胞運動の協奏」
  • Place of Presentation: 九州大学 箱崎キャンパス
  • Year and Date: 2014-12-19 – 2014-12-20
• [Presentation] フィードバックMRによる細胞骨格の力学挙動観測 (2014)
  • Author(s): 本田菜月, 西澤賢治, 有賀隆行, 柳島大輝, 水野大介
  • Organizer: 領域横断研究会「細胞力学と細胞運動の協奏
  • Place of Presentation: 九州大学箱崎キャンパス
  • Year and Date: 2014-12-19 – 2014-12-20
• [Presentation] フィードバックマイクロレオロジーを用いたソフトマターの力学挙動観測 (2014)
  • Author(s): 本田菜月, 西澤賢治, 有賀隆行, 柳島大輝, 水野大介
  • Organizer: 第120回日本物理学会九州支部例会
  • Place of Presentation: 崇城大学
  • Year and Date: 2014-12-06
• [Presentation] Investigation of the mechanism of cooperative cargo transport by multiple kinesins (2014)
  • Author(s): N. Sawairi, T. Ariga, M. Iwaki, M. Tomishige, K. Hayashi
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27