Study on effect of electrostatic interactions on stability of electron transfer complexes and amyloid fibrils
Project/Area Number |
25870407
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
Biophysics
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Research Institution | Osaka University |
Principal Investigator |
LEE Young-Ho 大阪大学, たんぱく質研究所, 講師 (70589431)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | Amyloid fibril / Binding thermodynamics / Calorimetry / Electron transfer / Electrostatics / Enzyme activity / Interprotein interaction / Protein misfolding / Charge interaction / Enthalpy and entropy / Hydrophobic effect / Intermolecular force / Protein aggregation / Thermodynamics |
Outline of Final Research Achievements |
We have conducted thermodynamic studies on binding reactions between ferredoxin (Fd) and sulfite reductase (SiR) as well as Fd and ferredoxin NADP(+)-reductase (FNR) using calorimetry, NMR spectroscopy, and X-ray crystallographic analysis with FNR and SiR activity assays. We have revealed that oppositely-charged Fd and SiR formed the electron transfer complex using attractive charge-charge interactions, and that non-electrostatic interactions was also important for fine tuning of binding modes between Fd and SiR to optimize SiR activity. The same results were also obtained in the binding system between Fd and FNR. We have also revealed that amyloid fibrils of alpha-synuclein cold-denatured below 293K due to the repulsive electrostatic interactions at low temperature. We have clearly demonstrated that interprotein electrostatic interactions play an important roles in normal protein functions and in clearance of disease-related amyloid fibrils.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Optimized Co-Solute Paramagnetic Relaxation Enhancement for the Rapid NMR Analysis of a Highly Fibrillogenic Peptide2015
Author(s)
Nur Alia Oktaviani, Michael W. Risor, Young-Ho Lee, Rik P. Megens, Djurre H. de Jong, Renee Otten, Ruud M. Scheek, Jan J. Enghild, Niels Chr. Nielsen, Takahisa Ikegami, and Frans A. A. Mulder
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Journal Title
Journal of Biomolecular NMR
Volume: ー
Pages: 印刷中
DOI
Related Report
Peer Reviewed
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[Journal Article] Ultrasonication-Dependent Formation and Degradation of Alpha-Synuclein Amyloid Fibrils2015
Author(s)
Hisashi Yagi, Aiko Mizuno, Masatomo So, Miki Hirano, Masayuki Adachi, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Tatsuya Ikenoue, Young-Ho Lee, Yasushi Kawata, and Yuji Goto
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Journal Title
Biochimica et Biophysica Acta
Volume: 1854
Pages: 209-217
DOI
Related Report
Peer Reviewed
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[Journal Article] NMR Characterization of the Interaction of the Endonuclease Domain of MutL with Divalent Metal Ions and ATP2014
Author(s)
Ryota Mizushima, Ju Yaen Kim, Isao Suetake, Hiroaki Tanaka, Tomoyo Takai, Narutoshi Kamiya, Yu Takano, Yuichi Mishima, Shoji Tajima, Yuji Goto, Kenji Fukui, and Young-Ho Lee
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Journal Title
PLoS ONE
Volume: 9
Pages: e98554
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of a New Interaction Mode between the Src Homology 2 (SH2) Domain of C-terminal Src Kinase (Csk) and Csk-Binding Protein (Cbp)/Phosphoprotein Associated with Glycosphingolipid Microdomains (PAG)2014
Author(s)
Hiroaki Tanaka(a), Ken-ichi Akagi, Chitose Oneyama, Masakazu Tanaka, Yuichi Sasaki, Takashi Kanou, Young-Ho Lee (b), Daisuke Yokogawa, Marc-Werner Dobenecker, Atsushi Nakagawa, Masato Okada, and Takahisa Ikegami
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Journal Title
Journal of biological chemistry
Volume: 288
Pages: 15240-15254
DOI
Related Report
Peer Reviewed
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[Journal Article] Structure, folding dynamics and amyloidogenesis of Asp76Asn β2-microglobulin: roles of shear flow, hydrophobic surfaces and α crystallin2013
Author(s)
P. Patrizia Mangione(a), Gennaro Esposito, Annalisa Relini, Sara Raimondi, Riccardo Porcari, Sofia Giorgetti, Alessandra Corazza, Federico Fogolari, Amanda Penco, Yuji Goto, Young-Ho Lee(b), Hisashi Yagi, Ciro Cecconi, Mohsin M. Naqvi, Julian D. Gillmore, Philip N. Hawkins, Fabrizio Chiti, Ranieri Rolandi et al
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Journal Title
Journal of biological chemistry
Volume: 288
Pages: 30917-30930
DOI
Related Report
Peer Reviewed
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