An investigation for degrees of reproductive isolation on the several contact zones among genetic lineages of Cynops pyrrhogaster

• Project/Area Number: 25870572
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics; Conservation of biological resources
• Research Institution: University of the Ryukyus
• Principal Investigator: TOMINAGA Atsushi 琉球大学, 教育学部, 准教授 (60452968)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生殖隔離 / 交雑帯 / 遺伝子浸透 / アカハライモリ / 分布境界 / 分類学的関係 / マイクロサテライト

Outline of Final Research Achievements

We detected nuclear genome differences between the two mtDNA clades of Cynops pyrrhogster. We found many hybrid individuals around the hybrid zone between the Central and the Western Japan groups in Chugoku district. On the contrary, we detected a few hybrid individuals around hybrid zone between the Northern and the Central Japan groups in Chubu district. The migration rate analysis revealed that an asymmetric gene introgression between two genetic groups in Chugoku district occurred, suggesting the possibility of range displacement of the two lineages as a consequence of the shift of their hybrid zone. On the contrary, we could not detected obvious nuclear genome differences based on microsatellite markers between the Tohoku and Kanto races in the Northern Japan group in Kanto district and between the Western and the Southern Japan groups in Kyushu district.

Research Products

• [Journal Article] Origin and Genetic Uniformity of Introduced Population of <i>Cynops pyrrhogaster</i> (Amphibia: Urodela) on Hachijojima Island (2016)
  • Author(s): Tominaga, A., Meyer-Rochow, V. B., Okamoto, T., Kuriyama, T., Nishikawa, K., Matsui, M.
  • Journal Title: Curr. Herpetol. Volume: 35 Issue: 1 Pages: 64-68
  • DOI: 10.5358/hsj.35.64
  • NAID: 130005128167
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Occurrence and Evolutionary History of Two <i>Cynops pyrrhogaster</i> Lineages on the Izu Peninsula (2015)
  • Author(s): Tominaga, A., Matsui, M., Kokuryo, Y.
  • Journal Title: Curr. Herpetol. Volume: 34 Issue: 1 Pages: 19-27
  • DOI: 10.5358/hsj.34.19
  • NAID: 130004888114
  • ISSN: 1345-5834, 1881-1019
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster. (2014)
  • Author(s): Tominaga A, Matsui M, Yoshikawa N, Hayano A, Inoue-Murayama M
  • Journal Title: Conservation Genetics Resources Volume: 6 Pages: 1-3
  • DOI: 10.1007/s12686-013-0104-5
  • Peer Reviewed / Open Access
• [Presentation] マイクロサテライト法によるシリケンイモリの親子判定の検討及びそれを用いた生活史の研究 (2016)
  • Author(s): 砂辺真之輔・富永篤
  • Organizer: 日本爬虫両棲類学会第55回大会
  • Place of Presentation: 琉球大学（沖縄県西原町）
  • Year and Date: 2016-11-26
• [Presentation] 中国地方におけるアカハライモリ２系統間の非対称な遺伝子浸透とそれに伴う交雑帯の移動 (2016)
  • Author(s): 富永篤・松井正文・吉川夏彦・江頭幸士郎・西川完途
  • Organizer: 日本爬虫両棲類学会第55回大会
  • Place of Presentation: 琉球大学（沖縄県西原町）
  • Year and Date: 2016-11-26
• [Presentation] Natural hybridization between two lineages of Japanese fire-bellied newt, Cynops pyrrhogaster in their contact zone (2016)
  • Author(s): Atsushi Tominaga, Masafumi Matsui, Natsuhiko Yoshikawa, Koshiro Eto, Kanto Nishikawa
  • Organizer: Joint Event: The 22nd International Congress of Zoology and the 89thAnnual Meeting of the Zoological Society of Japan
  • Place of Presentation: Okinawa Convension Center （沖縄県宜野湾市）
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] 分布境界におけるアカハライモリ系統間の遺伝関係 (2015)
  • Author(s): 富永篤・松井正文・西川完途・吉川夏彦・江頭幸士郎
  • Organizer: 日本動物学会第86回新潟大会
  • Place of Presentation: 新潟コンベンションセンター朱鷺メッセ
  • Year and Date: 2015-09-17
• [Presentation] 伊豆半島産のアカハライモリについて (2014)
  • Author(s): 富永篤・松井正文・国領康弘
  • Organizer: 日本爬虫両棲類学会 第 53 回大会
  • Place of Presentation: 神戸山手大学
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] 中国地方で側所的に分布するアカハライモリ2遺伝集団の形態学的な比較 (2013)
  • Author(s): 富永篤・松井正文
  • Organizer: 日本動物学会 第84回岡山大会
  • Place of Presentation: 岡山大学
• [Presentation] 中国地方で側所的に分布するアカハライモリ2群の遺伝的、形態学的比較 (2013)
  • Author(s): 富永篤・松井正文・吉川夏彦
  • Organizer: 日本爬虫両棲類学会 第52回大会
  • Place of Presentation: 東海大学札幌校
• [Remarks] 研究者データベース | 琉球大学
  • URL: http://kenkyushadb.lab.u-ryukyu.ac.jp/profile/ja.WC6JJ3jHaZcGESQ5odDdcQ==.html