Identification of anti-allergic compounds from Japanese pepper (Zanthoxylum piperitum) and elucidation of its mechanism
| Project/Area Number |
25870636
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
Biomolecular chemistry
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
Kono Ryohei 和歌山県立医科大学, 医学部, 助教 (70569110)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | アレルギー / 花粉症 / 脱顆粒 / 山椒 / 食品 / 抗アレルギー作用 / 脱顆粒抑制 / 肥満細胞 |
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| Outline of Final Research Achievements |
The aim of this study is to identify anti-allergic compounds from Japanese pepper (Zanthoxylum piperitum, ZP) and to elucidate the mechanism. Using famous rat mast cell line RBL-2H3, we screened the Japanese pepper extract on the basis of an inhibitory effect on a release of chemical mediator (degranulation) of IgE sensitized mast cell by antigen stimulation. As a result of this study, two inhibitory compounds were isolated from ZP. These two compounds attenuated intracellular Ca2+ mobilization induced by antigen stimulation. In addition, those inhibited the phosphorylation of Fyn related with migration of granule to the cell membrane, and the phosphorylation of Lyn which is upstream signal of intracellular Ca2+ mobilization. Furthermore, these compounds prevented membrane ruffling which is caused while degranulation.
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Report
(4 results)
Research Products
(2 results)
