Optimization of fusion gene identification methods

• Project/Area Number: 25871073
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics; Respiratory organ internal medicine
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: Togashi Yuki 公益財団法人がん研究会, 分子標的病理プロジェクト, 研究助手 (00648016)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: バイオマーカー / 融合遺伝子

Outline of Final Research Achievements

A) We compared lung tumors harboring fusion genes, EGFR or KRAS mutation, and those without the aberrations by global mRNA expression assay. Several highly-expressed genes were identified in ALK and ROS1 fusion-positive lung cancers. B) We developed an RNA capture sequencing system to detect the fusion genes and SNV/indels frequently observed in lung cancers. The system’s performance was excellent on both fresh frozen and formalin-fixed, paraffin-embedded specimens.

Research Products

• [Journal Article] Prospective and clinical validation of ALK immunohistochemistry: results from the phase I/II study of alectinib for ALK-positive lung cancer (AF-001JP study). (2016)
  • Author(s): Takeuchi K, Togashi Y, Kamihara Y, Fukuyama T, Yoshioka H, Inoue A, Katsuki H, Kiura K, Nakagawa K, Seto T, Maemondo M, Hida T, Harada M, Ohe Y, Nogami N, Yamamoto N, Nishio M, Tamura T.
  • Journal Title: Ann Oncol Volume: 27 Issue: 1 Pages: 185-192
  • DOI: 10.1093/annonc/mdv501
  • Peer Reviewed / Open Access
• [Journal Article] Mouse models for ROS1-fusion-positive lung cancers and their application to the analysis of multikinase inhibitor efficiency. (2016)
  • Author(s): Inoue M, Toki H, Matsui J, Togashi Y, Dobashi A, Fukumura R, Gondo Y, Minowa O, Tanaka N, Mori S, Takeuchi K, Noda T.
  • Journal Title: Carcinogenesis Volume: 37 Issue: 5 Pages: 452-460
  • DOI: 10.1093/carcin/bgw028
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Two Cases of Renal Cell Carcinoma Harboring a Novel STRN-ALK Fusion Gene (2016)
  • Author(s): Kusano H1, Togashi Y, Akiba J, Moriya F, Baba K, Matsuzaki N, Yuba Y, Shiraishi Y, Kanamaru H, Kuroda N, Sakata S, Takeuchi K, Yano H.
  • Journal Title: Am J Surg Pathol Volume: 40 Issue: 6 Pages: 761-769
  • DOI: 10.1097/pas.0000000000000610
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ALK-positive large B-cell lymphoma: identification of EML4-ALK and a review of the literature focusing on the ALK immunohistochemical staining pattern. (2016)
  • Author(s): Sakamoto K, Nakasone H, Togashi Y, Sakata S, Tsuyama N, Baba S, Dobashi A, Asaka R, Tsai CC, Chuang SS, Izutsu K, Kanda Y, Takeuchi K.
  • Journal Title: Int J Hematol Volume: 103 Issue: 4 Pages: 399-408
  • DOI: 10.1007/s12185-016-1934-1
  • Peer Reviewed / Open Access / Int'l Joint Research