Investigation of super-active TALEN in vitro
| Project/Area Number |
25871134
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Molecular biology
Laboratory animal science
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
ITO Yoko 独立行政法人理化学研究所, 生命システム研究センター, 特別研究員 (60584571)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | TALEN / ゲノム編集 / 人工ヌクレアーゼTALEN |
|---|
| Outline of Final Research Achievements |
Gene editing in vivo has become possible by the development of artificial nucleases that can be designed to cut the genome DNA selectively at the target site. TALENs are highly specific artificial nucleases. However in mammalian cells and embryos, TALENs often show poor activity. Recently we have developed “super-active” TALEN and demonstrated that which can mediate efficient genome editing in mouse embryos.
To evaluate how our “super-active” mutations actually affect enzymatic properties, we have produced active TALEN proteins and measured their activities in vitro. Our data suggested that super-active TALEN has high-affinity DNA-binding, which might cause high activity. In addition, structural biological approaches indicated the structural differences between super-active and conventional TALE. Our in vitro based approaches provide us a new insight for further improvement of TALEN techniques.
|
Report
(3 results)
Research Products
(4 results)
