| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We have already reported that the exocyst complex drives vesicle trafficking in adipocytes, a critical step in insulin stimulated glucose uptake (Inoue et al., Nature). In this study, we assessed the role of the exocyst complex in regulation of free fatty acid (FFA) uptake by adipocytes. Adipocytes incorporate extracellular FFAs in an insulin-dependent manner. A kinetic assay using fluoresceinated FFA (C12 dodecanoic acid) uptake allows the real-time monitoring of FFA internalization by adipocytes. The insulin-dependent uptake of C12 dodecanoic acid by adipocytes is mediated by Akt phosphorylation and microtubles. Knocking down of the exocyst components Exo70 and Sec8 significantly reduced insulin-dependent FFA uptake by adipocytes. These results demonstrate a novel role played by the exocyst complex in the regulation of FFA uptake by adipocytes.
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