| Project/Area Number |
25893220
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-08-30 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 歯根膜 / 血管内皮前駆細胞 / 血管新生 / 血管内皮細胞 / 血管周皮細胞 / 細胞分化 / 機械的応力 / シグナル伝達 |
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| Outline of Final Research Achievements |
We examined how contractile and suppressive forces affect the ability of periodontal ligament (PDL)-derived endothelial precursor cells (EPCs) to differentiate into endothelial cells (ECs) or pericytes. We found that contractile force against PDL-derived EPCs downregulated EC marker Tie-2 expression, but upregulated pericyte marker α-SMA expression. On the contrary, suppressive force against PDL-derived EPCs upregulated EC marker Tie-2 expression, but downregulated pericyte marker α-SMA expression. Thus, we succeeded to establish in vitro culture system by which we are able to evaluate how suppressive mechanical stress affects the multi-differentiation ability of these cells. We are trying to identify key genes to positively and negatively regulate differentiation of PDL-derived EPCs into ECs and perycytes.
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