Establishment of novel periodontal tissue regeneration using mesenchymal stem cells with high osteodifferentiation capacity

• Project/Area Number: 25893221
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontology
• Research Institution: Kansai Medical University (2014); Iwate Medical University (2013)
• Principal Investigator: SAWADA Shunsuke 関西医科大学, 医学部, 助教 (60711075)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: MSC / サイトカイン / 歯周病 / IL-1 / IL-6 / 再生医学 / 間葉系幹細胞 / トランスフェクション / インターロイキン / 再生医療

Outline of Final Research Achievements

Chronic periodontitis is an intraoral infectious disease that affects the nation with high prevalence. We see a lot of patients being forced to extract their teeth because of inflammatory bone resorption. To promote fundamental research of novel cell therapy using mesencymal stem cells, we established stem cell lines from the bone marrow of GFP transgenic mice and constructed a new expression vector targeting inhibition of several inflammatory cytokines. We expect that those new tools promote novel cell therapy in periodontal research.

Research Products

• [Journal Article] Establishment of mesenchymal stem cell lines derived from the bone marrow of GFP-transgenic mice exhibiting diversity in intracellular TGF-β and BMP signaling (2015)
  • Author(s): Shunsuke Sawada., Naoyuki Chosa., Naoki Takizawa., Jun Yokota., Yasuyuki Igarashi., Koichi Tomoda., Hisatomo Kondo., Takashi Yaegashi., Akira Ishisaki.
  • Journal Title: Molecular Medicine Reports Volume: -
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells. (2014)
  • Author(s): Aomatsu, E., Takahashi, N., Sawada, S., Okubo, N., Hasegawa, T., Taira, M., Miura, H., Ishisaki, A., and Chosa, N.
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 3652-3652
  • DOI: 10.1038/srep03652
  • Peer Reviewed / Open Access
• [Journal Article] PDGF-induced PI3K-mediated signal enhances TGF-βinduced osteogenic differentiation of human mesenchymal stem cells in the TGF-β-activated MEK-dependent manner (2014)
  • Author(s): Jun Yokota, Naoyuki Chosa, Shunsuke Sawada, Naoto Okubo, Noriko Takahashi, Tomokazu Hasegawa, Hisatomo Kondo, Akira Ishisaki
  • Journal Title: International Journal of Molecular Medicine Volume: 33 Issue: 3 Pages: 534-542
  • DOI: 10.3892/ijmm.2013.1606
  • Peer Reviewed / Open Access
• [Journal Article] PDGF-induced PI3K-mediated signal enhances TGF-β-induced osteogenic differentiation of human mesenchymal stem cells in the TGF-β-activated MEK-dependent manner (2014)
  • Author(s): Yokota J, Chosa N, Sawada S, Okubo N, Takahashi N, Hasegawa T, Kondo H, Ishisaki A.
  • Journal Title: Int J Mol Med Volume: 33(3) Pages: 534-42
  • Peer Reviewed
• [Presentation] GFPマウス骨髄由来間葉系幹細胞の分化能とサイトカイン受容体の発現 (2014)
  • Author(s): 滝沢尚希、澤田俊輔、伊東俊太郎、佐々木大輔、帖佐直幸、石崎明、八重柏隆
  • Organizer: 第140回日本歯科保存学会春期学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール（滋賀）
  • Year and Date: 2014-06-20
• [Presentation] GFPマウス骨髄由来間葉系幹細胞の分化能と網羅的遺伝子解析 (2014)
  • Author(s): 澤田俊輔、兒島由佳、友田幸一
  • Organizer: 第59会回日本口腔外科学会総会・学術大会
  • Place of Presentation: 幕張メッセ（千葉）
  • Year and Date: 2014-06-17
• [Presentation] 口腔ケア処置前後による口腔内細菌数の変動について (2014)
  • Author(s): 河合崇普、村井治、金澤智美、澤田俊輔、相羽健太郎、藤原英明、水城まさみ、菊池善博、八重柏隆
  • Organizer: 第57回日本歯周病学会春期学術大会
  • Place of Presentation: 長良川国際会議場（岐阜）
  • Year and Date: 2014-05-23
• [Presentation] GFPマウス骨髄由来間葉系幹細胞の株化とサイトカイン関連遺伝子群の発現解析 (2014)
  • Author(s): 澤田俊輔、伊東俊太郎、佐々木大輔、藤原英明、村井治、八重柏隆
  • Organizer: 第57回日本歯周病学会春期学術大会
  • Place of Presentation: 長良川国際会議場（岐阜）
  • Year and Date: 2014-05-23
• [Presentation] Development of Novel Cell Therapy Using Mesencymal Stem Cells in Periodontitis
  • Author(s): Shunsuke Sawada
  • Organizer: Harvard University School of Dentistry Iwate Medical University School of Dentistry exchanging program, International Student Forum 2013
  • Place of Presentation: Morioka, Japan
• [Presentation] Establishment of a novel cell therapy for periodontitis using MSCs enhanced osteogenic differentiation and anti-inflammation capacities
  • Author(s): Shunsuke Sawada
  • Organizer: IADR Hatton Awards国内二次選考会
  • Place of Presentation: Tokyo, Japan
  • Invited
• [Presentation] 培養歯髄細胞におけるIL-6刺激伝達経路
  • Author(s): 藤原英明、澤田俊輔、伊東俊太郎、金澤智美、大川義人、佐々木大輔、村井治、八重柏隆
  • Organizer: 第139回日本歯科保存学会秋期学術大会
  • Place of Presentation: 秋田
• [Presentation] 歯肉線維芽細胞のマトリックスメタロプロテアーゼ発現を制御する細胞内シグナル伝達
  • Author(s): 滝沢尚希, 澤田俊輔, 伊東俊太郎, 大川義人, 金澤智美, 佐々木大輔, 藤原英明, 村井治, 石崎明, 八重柏隆
  • Organizer: 第56回秋期日本歯周病学会学術大会
  • Place of Presentation: 前橋