3D dynamic imaging of gliding-srcretion apparatus in Bacteroidetes

• Project/Area Number: 25893230
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including mycology)
• Research Institution: Gakushuin University
• Principal Investigator: NAKANE Daisuke 学習院大学, 理学部, 助教 (40708997)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: バクテリア / 滑走運動 / 分泌装置 / らせん / 光学顕微鏡 / 無限軌道 / フラボバクテリア / キャタピラ / 3次元 / 分子モーター

Outline of Final Research Achievements

Cells of Flavobacterium johnsoniae crawl rapidly over surfaces in a process called gliding motility. These cells do not have flagella or pili but instead rely on a novel motility machine composed of proteins that are unique to the phylum Bacteroidetes. The motility adhesins SprB and RemA are propelled along helical paths on the cell surface by the still poorly-defined gliding motor. Interaction of these adhesins with a surface results in rotation and translocation of the cell. SprB and RemA are delivered to the cell surface by the type IX secretion system (T9SS). T9SSs are confined to but common in the phylum Bacteroidetes. Transmembrane components of the T9SS may perform roles in both secretion and gliding motility.

Research Products

• [Journal Article] Bacteriocin Protein BacL1 of Enterococcus faecalis Targets CelDivision Loci and Specifically Recognizes L-Ala2-Cross-Bridged Peptidoglycan (2015)
  • Author(s): Jun Kurushima, Daisuke Nakane, Takayuki Nishizaka, Haruyoshi Tomitaa
  • Journal Title: Juornal of Bacteriology Volume: 197 Issue: 2 Pages: 286-295
  • DOI: 10.1128/jb.02203-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 細菌滑走運動の新しいメカニズム~戦車のような仕組みで動くバクテリア (2015)
  • Author(s): 中根大介、中山浩次、西坂崇之
  • Journal Title: 生物と化学 Volume: 53 Pages: 215-216
  • Peer Reviewed
• [Journal Article] Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system. (2014)
  • Author(s): 7.Narita Y, Sato K, Yukitake H, Shoji M, Daisuke N, Nagano K, Yoshimura F, Naito M, Nakayama K
  • Journal Title: Microbiology-SGM Volume: 160 Issue: 10 Pages: 2295-2303
  • DOI: 10.1099/mic.0.080192-0
  • NAID: 120006986357
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Unitary step of gliding machinery in Mycoplasma mobile. (2014)
  • Author(s): Kinosita Y, Nakane D, Sugawa M, Masaike T, Mizutani K, Miyata M, Nishizaka T.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 111 Issue: 23 Pages: 8601-6
  • DOI: 10.1073/pnas.1310355111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 戦車のような仕組みで動くバクテリア (2014)
  • Author(s): 中根大介、中山浩次、西坂崇之
  • Journal Title: 生物物理 Volume: 54 Pages: 269-270
  • NAID: 130004693956
  • Peer Reviewed / Open Access
• [Journal Article] Helical flow of surface protein required for bacterial gliding motility. (2013)
  • Author(s): 102. Nakane D, Sato K, Wada H, McBride MJ, Nakayama K
  • Journal Title: Proc. Nat. Acd. Sci. USA Volume: 110 Issue: 27 Pages: 11145-11150
  • DOI: 10.1073/pnas.1219753110
  • Peer Reviewed
• [Journal Article] Bidirectional bacterial gliding motility powered by the collective transport of cell surface proteins (2013)
  • Author(s): Hirofumi Wada, Daisuke Nakane, Hsuan-Yi Chen
  • Journal Title: Physical Review Letters Volume: 111 Issue: 24 Pages: 248102-248102
  • DOI: 10.1103/physrevlett.111.248102
  • Peer Reviewed
• [Presentation] IX型分泌装置を可視化する (2015)
  • Author(s): 中根大介
  • Organizer: 第88回 日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
  • Invited
• [Presentation] バクテロイデーテス細菌がもつ滑走・分泌装置の構造ダイナミクス (2014)
  • Author(s): 中根大介、中山浩次、西坂崇之
  • Organizer: 細菌学会関東支部会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-30 – 2014-10-31
• [Presentation] 原核生物の体の動かし方 (2014)
  • Author(s): 中根大介
  • Organizer: 環境微生物系学会合同大会 2014
  • Place of Presentation: 静岡
  • Year and Date: 2014-10-22 – 2014-10-24
  • Invited
• [Presentation] 左にしか曲がれないバクテリア (2014)
  • Author(s): 中根大介、西坂崇之
  • Organizer: 第8回 細菌学若手コロッセウム
  • Place of Presentation: 北海道
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] Bacterium moves like a tank (2014)
  • Author(s): 中根大介
  • Organizer: 第37回 内藤カンファレンス
  • Place of Presentation: 北海道
  • Year and Date: 2014-07-15 – 2014-07-18
• [Presentation] 戦車のような仕組みで動くバクテリア (2014)
  • Author(s): 中根大介
  • Organizer: 日本農芸化学会 2014年度大会
  • Place of Presentation: 神奈川
  • Invited
• [Presentation] 戦車のような仕組みで動くバクテリア (2014)
  • Author(s): 中根大介、佐藤啓子、中山浩次、西坂崇之
  • Organizer: 第87回 日本細菌学会 総会
  • Place of Presentation: 東京
• [Presentation] 滑走するバクテリアがつくる巨大渦パターン (2014)
  • Author(s): 中根大介、小高祥子、西坂崇之
  • Organizer: 2013年度 べん毛交流会
  • Place of Presentation: 広島
• [Presentation] 戦車のような仕組みで動くバクテリア (2013)
  • Author(s): 中根大介、佐藤啓子、和田浩史、Mark J. McBride、中山浩次、西坂崇之
  • Organizer: 第29回 日本微生物生態学会 大会
  • Place of Presentation: 鹿児島
• [Book] Mollicutes: Molecular Biology and Pathogenesis (2014)
  • Author(s): Makoto Miyata and Daisuke Nakane
  • Total Pages: 324
  • Publisher: Horizon Press
• [Remarks] 学習院大学 プレスリリース
  • URL: http://www.gakushuin.ac.jp/ad/kikaku/koho/media/20140523.html
• [Remarks] 産経新聞 ニュース
  • URL: http://www.sankei.com/life/news/140804/lif1408040023-n1.html
• [Remarks] バクテリアの滑走運動のメカニズムの解明
  • URL: http://www.nagasaki-u.ac.jp/ja/about/info/science/science56.html