The development of environmentally responsive fluorescence polymer probes for visualizing pathological cells
Project/Area Number |
25893232
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Physical pharmacy
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Research Institution | Keio University |
Principal Investigator |
HIRUTA YUKI 慶應義塾大学, 薬学部, 助教 (60710944)
|
Project Period (FY) |
2013-08-30 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 温度応答性高分子 / 高分子合成 / 機能性蛍光プローブ / ナノ材料 / 細胞イメージング / がん細胞 / 環境応答性高分子 |
Outline of Final Research Achievements |
The discrimination of normal and pathological cells is attractive from a clinical viewpoint. Tumor cells might have higher temperatures than normal ones as a result of enhanced metabolic activities. In addition, tumorous extracellur pH is slightly lower than that of normal tissue. Therefore, We developed poly(N-isopropylacrylamide)-based temperature-responsive fluorescence polymer probe, and pH-responsive fluorescence polymer probe. The cellular uptake of temperature-responsive fluorescence polymer probe can be controlled within only 1℃. pH-Responsive fluorescence polymer probe exhibited a specific intracellular uptake only in weak acidic conditions. The ability for temperature-responsive and acidic environment selective intracellular uptake opens up the visualization of selective tumors at the cellular level.
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Report
(3 results)
Research Products
(21 results)