Prevention and treatment for sensorineural hearing loss by controlling the system of innate immunity

• Project/Area Number: 25893299
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: 独立行政法人国立病院機構(東京医療センター臨床研究センター)
• Principal Investigator: HAYASHI Yushi 独立行政法人国立病院機構(東京医療センター臨床研究センター), 政策医療企画研究部, 医師 (40715166)
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 蝸牛 / 自然免疫 / 有毛細胞 / 支持細胞 / 感音難聴 / ウイルス感染 / I型IFN / RIG-I family / Ｉ型IFN

Outline of Final Research Achievements

We analyzed the mechanism of sensorineural hearing loss caused by viral infection such as sudden hearing loss, Meniere's disease and congenital hearing loss using ex vivo system of cochlear sensory epithelia. To investigate the influence of type I interferon (IFN) which is transcribed in supporting cells during viral infection on the cochlear sensory epithelium, we used IFNAR1 which is a receptor of type I IFN knock out mice.

Research Products

• [Journal Article] Insulin-like growth factor 1 induces the transcription of Gap43 and Ntn1 during hair cell protection in the neonatal murine cochlea. (2014)
  • Author(s): Hayashi Y, Yamamoto N, Nakagawa T, Ito J.
  • Journal Title: Neurosci Lett. Volume: 560 Pages: 7-11
  • DOI: 10.1016/j.neulet.2013.11.062.
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor 1 inhibits hair cell apoptosis and promotes the cell cycle of supporting cells by activating different downstream cascades after pharmacological hair cell injury in neonatal mice. (2013)
  • Author(s): Hayashi Y, Yamamoto N, Nakagawa T, Ito J.
  • Journal Title: Mol Cell Neurosci. Volume: 56 Pages: 29-38
  • DOI: 10.1016/j.mcn.2013.03.003.
  • Peer Reviewed
• [Journal Article] Virus-induced expression of retinoic acid inducible gene-I and melanoma differentiation-associated gene 5 in the cochlear sensory epithelium. (2013)
  • Author(s): Hayashi Y, Onomoto K, Narita R, Yoneyama M, Kato H, Nakagawa T, Ito J, Taura A, Fujita T.
  • Journal Title: Microbes Infect. Volume: 15 Pages: 592-598
  • DOI: 10.1016/j.micinf.2013.04.008.
  • Peer Reviewed
• [Presentation] The Pathogenesis of Sensorineural Hearing Loss During Virus Infection of the Cochlear Sensory Epithelium (2015)
  • Author(s): Yushi Hayashi, Koji Onomoto, Mitsutoshi Yoneyama, Kimitaka Kaga, Takashi Fujita, Nobuyuki Tanaka
  • Organizer: 38th Association for Research in Otolaryngology MidWinter Meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2015-02-21 – 2015-02-25
• [Presentation] Type I IFN is produced in supporting cells against virus infection of the cochlear sensory epithelium via RIG-I like receptor signaling pathway. (2014)
  • Author(s): Hayashi Y, Onomoto K, Narita R, Yoneyama M, Kato H, Taura A, Nakagawa T, Ito J, Fujita T, Kaga K.
  • Organizer: ARO 37th MidWinter Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20140222-20140226
• [Presentation] Type I IFN is produced in supporting cells against virus infection of the cochlear sensory epithelium via RIG-I like receptor signaling pathway (2014)
  • Author(s): Yushi Hayashi, Koji Onomoto, Ryo Narita, Mitsutoshi Yoneyama, Hiroki Kato, Akiko Taura, Takayuki Nakagawa, Juichi Ito, Kimitaka Kaga, Takashi Fujita
  • Organizer: Inner Ear Biology 2014 in Kyoto
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-11-01 – 2014-11-04
• [Presentation] Type I IFN is produced in supporting cells against virus infection of the cochlear sensory epithelium via RIG-I like receptor signaling pathway (2014)
  • Author(s): Yushi Hayashi, Koji Onomoto, Ryo Narita, Mitsutoshi Yoneyama, Hiroki Kato, Akiko Taura, Takayuki Nakagawa, Juichi Ito, Kimitaka Kaga, Takashi Fujita
  • Organizer: 51st Inner Ear Biology Workshop
  • Place of Presentation: Sheffield, UK
  • Year and Date: 2014-08-30 – 2014-09-02
• [Presentation] 全身III度の熱傷に対する加療で薬剤性に両耳聾となり、人工内耳埋め込み手術を実施するに至った成人の1例 (2013)
  • Author(s): 林 裕史、竹腰英樹、加我君孝
  • Organizer: 第23回日本耳科学会総会
  • Place of Presentation: 宮崎市
  • Year and Date: 20131124-20131126
• [Book] Microtia and Atretia. Temporal bone computed tomography of patients with poor postoperative hearing improvement after surgery for atresia of the external auditory canal. (2014)
  • Author(s): Hayashi Y, Kaga K.
  • Total Pages: 4
  • Publisher: Karger