王 英正 岡山大学, 大学病院, 教授 (50372579)
高橋 賢 岡山大学, 医歯薬学総合研究科, 助教 (50432258)
松浦 宏治 岡山理科大学, 工学部, 准教授 (70443223)
入部 玄太郎 岡山大学, 医歯薬学総合研究科, 准教授 (90284885)
|Budget Amount *help
¥201,760,000 (Direct Cost: ¥155,200,000、Indirect Cost: ¥46,560,000)
Fiscal Year 2018: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2017: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2016: ¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000)
Fiscal Year 2015: ¥53,170,000 (Direct Cost: ¥40,900,000、Indirect Cost: ¥12,270,000)
Fiscal Year 2014: ¥56,030,000 (Direct Cost: ¥43,100,000、Indirect Cost: ¥12,930,000)
|Outline of Final Research Achievements
We induced differentiation of human induced pluripotent stem cells into spontaneously beating cardiomyocytes. We elucidated that the cardiac differentiation is facilitated by cyclic stretch stimulus and co-culture with human fibroblasts. It is suggested that stretch stimulus to cardiac stem cells induces release of paracrine factors, thereby increase cardiac contractility. With regard to autotransplantation of cardiac stem cells in pediatric dilated cardiomyopathy, we conducted the TICAP-DCM phase I trial and performed surgery for three patients successfully.
We also studied the effect of mechanical stimulus on gene expression of fertilized eggs in mice and found that the expression of genes related to embryo development and cell death was altered in response to mechanical stimulus. In addition, we successfully developed a high-quality silicone resin incubation chamber for human fertilized eggs. Furthermore, we found that hydrostatic pressure activates sperm activity.