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Characterization of molecular evolution of plant isoquinoline alkaloid biosynthesis and its application to metabolic engineering

Research Project

Project/Area Number 26221201
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

Sato Fumihiko  京都大学, 生命科学研究科, 教授 (10127087)

Co-Investigator(Kenkyū-buntansha) 南 博道  石川県立大学, 公私立大学の部局等, 准教授 (90433200)
細江 智夫  星薬科大学, 薬学部, 教授 (10287849)
山田 泰之  神戸薬科大学, 薬学部, 助教 (20770879)
Co-Investigator(Renkei-kenkyūsha) NAGAO Masaya  京都大学, 生命科学研究科, 教授 (10237498)
KAWANO Noriaki  (独)医薬基盤研究所, 薬用植物資源研究センター, 主任研究員 (70392298)
YAMATO Katsuyuki  近畿大学, 生物理工学部, 教授 (50293915)
NISHIDA Ritsuo  京都大学, 農学研究科, 教授 (30135545)
HIRAKAWA Hideki  かずさDNA研究所, ゲノム情報解析グループ, グループ長 (80372746)
Research Collaborator TOYODA Atsushi  国立遺伝学研究所, 教授 (10267495)
Project Period (FY) 2014-05-30 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥186,030,000 (Direct Cost: ¥143,100,000、Indirect Cost: ¥42,930,000)
Fiscal Year 2017: ¥40,950,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥9,450,000)
Fiscal Year 2016: ¥40,950,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥9,450,000)
Fiscal Year 2015: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2014: ¥58,240,000 (Direct Cost: ¥44,800,000、Indirect Cost: ¥13,440,000)
Keywords植物アルカロイド生合成系 / 生合成系の分子進化 / 代謝工学と代謝再構築 / 有用二次代謝産物生産 / 生理機能評価
Outline of Final Research Achievements

1) Genes for biosynthetic enzymes, especially novel cytochrome P450 and O-methyltransferases, and transcription factors in isoquinoline alkaloid (IQA) biosynthesis were identified based on the draft genome sequence and trascriptome analysis of Eschscholzia californica. 2) Post-transcriptional regulation of WRKY transcription factor in Coptis japonica were characterized for the stable metabolite production. 3) Transcriptomes were characterized in several IQA producing plants to isolate IQA biosynthetic enzyme genes. 4) Common origin of methylenedioxy ring degradation and demethylation enzyme was identified in bacteria. 5) For the microbial production of useful IQAs, total biosynthesis of opiates by stepwise fermentation was established in engineered Escherichia coli, beside the efficient microbial production of stylopine using a Pichia pastoris expression system. 6) 13-Methylberberine, a berberine analogue with stronger anti-adipogenic effects on mouse 3T3-L1 cells was identified.

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(9 results)
  • 2018 Research Progress Assessment (Verification Result) ( PDF )
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2015 Annual Research Report
  • 2014 Abstract ( PDF )   Annual Research Report
  • Research Products

    (84 results)

All 2018 2017 2016 2015 2014 Other

All Journal Article (24 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 24 results,  Open Access: 14 results,  Acknowledgement Compliant: 8 results) Presentation (50 results) (of which Int'l Joint Research: 10 results,  Invited: 14 results) Book (1 results) Remarks (9 results)

  • [Journal Article] Mining of the Uncharacterized Cytochrome P450 Genes Involved in Alkaloid Biosynthesis in California Poppy Using a Draft Genome Sequence2018

    • Author(s)
      Hori Kentaro、Yamada Yasuyuki、Purwanto Ratmoyo、Minakuchi Yohei、Toyoda Atsushi、Hirakawa Hideki、Sato Fumihiko
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Issue: 2 Pages: 222-233

    • DOI

      10.1093/pcp/pcx210

    • NAID

      120006384165

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The function of <i>ETHYLENE RESPONSE FACTOR</i> genes in the light-induced anthocyanin production of <i>Arabidopsis thaliana</i> leaves2018

    • Author(s)
      T. Koyama, F. Sato
    • Journal Title

      Plant Biotechnology

      Volume: 35 Issue: 1 Pages: 87-91

    • DOI

      10.5511/plantbiotechnology.18.0122b

    • NAID

      130006727685

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microbial production of novel sulphated alkaloids for drug discovery2018

    • Author(s)
      Matsumura E., Nakagawa A., Tomabechi Y., Ikushiro S., Sakaki T., Katayama T., Yamamoto K., Kumagai H., Sato F. and Minami H.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-018-26306-7

    • NAID

      120006517899

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dihydrosanguinarine Enhances Glucose Uptake in Mouse 3T3-L1 Cells2017

    • Author(s)
      Yit-Lai Chow, Yuko Iwata and Fumihiko Sato
    • Journal Title

      ACS omega

      Volume: 2 Issue: 10 Pages: 6916-6925

    • DOI

      10.1021/acsomega.7b01134

    • NAID

      120006355765

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modulation of benzylisoquinoline alkaloid biosynthesis by heterologous expression of CjWRKY1 in Eschscholzia californica cells2017

    • Author(s)
      Yasuyuki Yamada, Tomoe Shimada, Yukiya Motomura, Fumihiko Sato
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 10 Pages: 186953-186955

    • DOI

      10.1371/journal.pone.0186953

    • NAID

      120006358795

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of miR319 and TCP transcription factors in leaf development2017

    • Author(s)
      Koyama Tomotsugu、Sato Fumihiko、Ohme-Takagi Masaru
    • Journal Title

      Plant Physiology

      Volume: 175 Issue: 2 Pages: 874-885

    • DOI

      10.1104/pp.17.00732

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unraveling Additional O-Methylation Steps in Benzylisoquinoline Alkaloid Biosynthesis in California Poppy (Eschscholzia californica)2017

    • Author(s)
      Purwanto Ratmoyo、Hori Kentaro、Yamada Yasuyuki、Sato Fumihiko
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 9 Pages: 1528-1540

    • DOI

      10.1093/pcp/pcx093

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Common origin of methylenedioxy ring degradation and demethylation in bacteria2017

    • Author(s)
      Takeda Hisashi、Ishikawa Kazuki、Yoshida Hinaka、Kasai Daisuke、Wakana Daigo、Fukuda Masao、Sato Fumihiko、Hosoe Tomoo
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 7422-7422

    • DOI

      10.1038/s41598-017-07370-x

    • NAID

      120006358777

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cloning and Characterization of Cheilanthifoline and Stylopine Synthase Genes from Chelidonium majus2017

    • Author(s)
      Yahyazadeh Mahdi、Ratmoyo Purwanto、Bittner Florian、Sato Fumihiko、Selmar Dirk
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 8 Pages: 1421-1430

    • DOI

      10.1093/pcp/pcx077

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A multidrug and toxic compound extrusion transporter mediates berberine accumulation into vacuoles in Coptis japonica2017

    • Author(s)
      Takanashi K., Yamada Y., Sasaki T., Yamamoto Y., Sato F., Yazaki K.
    • Journal Title

      Phytochemistry

      Volume: 138 Pages: 76-82

    • DOI

      10.1016/j.phytochem.2017.03.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laboratory-scale production of (S)-reticuline, an important intermediate of benzylisoquinoline alkaloids, using a bacterial-based method2017

    • Author(s)
      Eitaro Matsumura, Akira Nakagawa, Yusuke Tomabechi, Takashi Koyanagi, Hidehiko Kumagai, Kenji Yamamoto, Takane Katayama, Fumihiko Sato, and Hiromichi Minami
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 81 Issue: 2 Pages: 396-402

    • DOI

      10.1080/09168451.2016.1243985

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 13-Methylberberine, a berberine analogue with stronger anti-adipogenic effects on mouse 3T3-L1 cells2016

    • Author(s)
      Yit-Lai Chow, Mami Sogame and Fumihiko Sato
    • Journal Title

      Scientific Rep

      Volume: 6 Issue: 1 Pages: 38129-38129

    • DOI

      10.1038/srep38129

    • NAID

      120005954471

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of the promoter region of biosynthetic enzyme genes involved in berberine biosynthesis in Coptis japonica2016

    • Author(s)
      Yasuyuki Yamada, Tadashi Yoshimoto, Sayumi Tsushima Yoshida and Fumihiko Sato
    • Journal Title

      Frontiers in Plant Science

      Volume: 7 Pages: 1352-1352

    • DOI

      10.3389/fpls.2016.01352

    • NAID

      120005980813

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tyrosine phosphorylation and protein degradation control the transcriptional activity of WRKY involved in benzylisoquinoline alkaloid biosynthesis2016

    • Author(s)
      Yamada, Y., Sato, F.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 31988-31988

    • DOI

      10.1038/srep31988

    • NAID

      120005829416

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of shikonin derivative secretion in Lithospermum erythrorizon hairy roots as a model of lipid-soluble metabolite secretion from plants2016

    • Author(s)
      Kanade Tasumi, Mariko Yano, Kenta Kaminade, Akifumi Sugiayama, Mayuko Sato, Kiminori Toyooka, Takashi Aoyama, Fumihiko Sato, and Kazufumi Yazaki
    • Journal Title

      Frontier in Plant Science

      Volume: 7 Pages: 1066-1066

    • DOI

      10.3389/fpls.2016.01066

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli.2016

    • Author(s)
      Nakagawa A., Matsumura E., Koyanagi T., Katayama T., Kawano N., Yoshimatsu K., Yamamoto K., Kumagai H., Sato F. and Minami H.
    • Journal Title

      Nature Communications

      Volume: 7 Issue: 1 Pages: 10390-10390

    • DOI

      10.1038/ncomms10390

    • NAID

      120005702806

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficient microbial production of stylopine using a Pichia pastoris expression system.2016

    • Author(s)
      Hori, K., Okano, S., Sato F.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 22201-22201

    • DOI

      10.1038/srep22201

    • NAID

      120005819484

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Isolation and identification of berberine and berberrubine metabolites by berberine-utilizing bacterium, Rhodococcus sp. strain BD71002016

    • Author(s)
      Ishikawa, K., Takeda, H., Wakana, D., Sato F.and Hosoe, T.
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 80 Issue: 5 Pages: 856-862

    • DOI

      10.1080/09168451.2015.1136878

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 11-Hydroxylation of protoberberine by the novel berberine-utilizing aerobic bacterium Sphingobium sp. strain BD31002015

    • Author(s)
      Takeda, H., Ishikawa, K., Wakana, D., Fukuda, M., Sato F., Hosoe, T.
    • Journal Title

      J. Nat. Prod.

      Volume: 78(12) Issue: 12 Pages: 2880-2886

    • DOI

      10.1021/acs.jnatprod.5b00408

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two B-type ATP-binding cassette (ABC) transporters localize to the plasma membrane in <i>Thalictrum minus</i>2015

    • Author(s)
      Shitan, N., Terasaka, K., Yamamoto, H., Sato F., Yazaki, K.
    • Journal Title

      Plant Biotechnology

      Volume: 32 Issue: 3 Pages: 243-247

    • DOI

      10.5511/plantbiotechnology.15.0604a

    • NAID

      130005105922

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CjbHLH1 homologs regulate sanguinarine biosynthesis in Eschscholzia californica cells2015

    • Author(s)
      Yamada, Y., Motomura, Y., Sato, F.
    • Journal Title

      Plant Cell Physiol.

      Volume: 56 Issue: 5 Pages: 1019-1030

    • DOI

      10.1093/pcp/pcv027

    • NAID

      120005593599

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] (R,S)-Tetrahydropapaveroline Production by Stepwise Fermentation Using Engineered Escherichia coli.2014

    • Author(s)
      A. Nakagawa, C. Matsuzaki, E. Matsumura, T. Koyanagi, T. Katayama, K. Yamamoto, F. Sato, H. Kumagai, and H. Minami
    • Journal Title

      Sci. Rep.

      Volume: 4:6695 Issue: 1 Pages: 1-8

    • DOI

      10.1038/srep06695

    • NAID

      120006358778

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Knockdown of the NHR-8 Nuclear Receptor Enhanced Sensitivity to the Lipid-Reducing Activity of Alkaloids in Caenorhabditis elegans.2014

    • Author(s)
      Y.-L. Chow, Y. Kawasaki, and F. Sato
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 78 Issue: 12 Pages: 2008-2013

    • DOI

      10.1080/09168451.2014.940278

    • NAID

      120005601058

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Asymmetric Synthesis of Tetrahydro- isoquinolines by Enzymatic Pictet-Spengler Reaction2014

    • Author(s)
      Nishihachijo, M., Hirai, Y., Kawano, S., Nishiyama, A., Minami, H., Katayama, T., Yasohara, Y., Sato, F., Kumagai, H.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 78 Issue: 4 Pages: 701-707

    • DOI

      10.1080/09168451.2014.890039

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] ハナビシソウにおけるイソキノリンアルカロイド生合成遺伝子の発現ネットワーク解析2018

    • Author(s)
      西田昇平、山田泰之、佐藤文彦
    • Organizer
      2018年度日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウマノスズクサにおけるアリストロキア酸生合成酵素遺伝子の探索と機能解析(II)2018

    • Author(s)
      松井俊樹、佐藤文彦
    • Organizer
      2018年度日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR/Cas9 systemを用いたオウレンゲノム編集細胞の作成と一細胞解析2018

    • Author(s)
      浜 竜哉、西尾 洋、山田泰之、佐藤文彦
    • Organizer
      2018年度日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] オウレンのイソキノリンアルカロイド生合成系を制御するAP2/ERF型転写因子の機能解析2018

    • Author(s)
      山田泰之、西田昇平、士反伸和、佐藤文彦
    • Organizer
      2018年度日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アルカロイド耐性・輸送体タンパク質を用いた効率的なアルカロイド生産系の検討2018

    • Author(s)
      炭田奈々、山田泰之、大木秀浩、松井治幸、潤井みや、南博道、佐藤文彦、士反伸和
    • Organizer
      2018年日本薬学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synthetic biology of plant secialized metabolism using NGS information of non-model medicinal plants2017

    • Author(s)
      Fumihiko Sato
    • Organizer
      Plant Genomics & Gene Editing, Asia Congress
    • Place of Presentation
      Eaton Hong Kong
    • Year and Date
      2017-04-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 創薬研究を目指した大腸菌を用いたベンジルイソキノリンアルカロイド生産系の構築2017

    • Author(s)
      中川 明・佐藤文彦・南 博道
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      東北大学
    • Year and Date
      2017-03-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 微生物発酵法による植物アルカロイド生産と生薬生理活性物質の創製2017

    • Author(s)
      南 博道
    • Organizer
      産学官学術交流フォーラム. 日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] イソキノリンアルカロイド生合成系の発現制御に関わるAP2/ERF型転写因子群の探索2017

    • Author(s)
      山田 泰之, 西田昇平, 佐藤文彦
    • Organizer
      2017年度日本農芸化学会大会(京都)
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] CRISPR/Cas9 systemを用いたオウレンのゲノム編集2017

    • Author(s)
      浜 竜哉、西尾 洋、山田 泰之、佐藤 文彦
    • Organizer
      2017年度日本農芸化学会大会(京都)
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] ウマノスズクサにおけるアリストロキア酸生合成酵素遺伝子の探索と機能同定2017

    • Author(s)
      松井俊樹, 堀健太郎, 山田泰之, 佐藤文彦
    • Organizer
      2017年度日本農芸化学会大会(京都)
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Microbial production of opiates and related Isoquinoline alkaloids2017

    • Author(s)
      Fumihiko Sato
    • Organizer
      16th Annual PEPTalk, Microbial Production; Optimizing the Production of Microbial-based Expression Systems
    • Place of Presentation
      Hilton San Diego Bayfront, San Diego, CA
    • Year and Date
      2017-01-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sphingobium sp.BD3100株のベルベリン脱メチレン化酵素の誘導性2017

    • Author(s)
      武田尚、若菜大悟、宮内啓介、細江智夫
    • Organizer
      環境微生物系学会合同大会2017(仙台)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ベルベリン資化性菌Rhodococcus sp. BD7100株がオウレンエキス添加培養時に産生する抗菌物質に関する検討2017

    • Author(s)
      石川和樹、吉田陽菜花、武田尚、若菜大悟、東條元昭、佐藤文彦、細江智夫
    • Organizer
      環境微生物系学会合同大会2017(仙台)
    • Related Report
      2017 Annual Research Report
  • [Presentation] イソキノリンアルカロイド生合成系の制御に関わるAP2/ERF転写因子群の探索と機能解析2017

    • Author(s)
      山田 泰之, 西田昇平, 佐藤文彦
    • Organizer
      第35回日本植物細胞分子生物学会 (さいたま) 大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Synthetic biology goes in benzylisoquinoline alkaloid biosynthesis2017

    • Author(s)
      Fumihiko Sato
    • Organizer
      The 4th International Conference on Plant Metabolism (ICPM 2017), Dalian, China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Synthetic biology of plant secialized metabolism using NGS information of non-model medicinal plants2017

    • Author(s)
      Fumihiko Sato
    • Organizer
      Plant Genomics & Gene editing, Asia Congress, Eaton Hong Kong
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 創薬研究を目指したベンジルイソキノリンアルカロイドの大腸菌を用いた生産系の構築2017

    • Author(s)
      中川 明、佐藤 文彦、片山 高嶺、南 博道
    • Organizer
      日本生物工学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 微生物発酵法による生薬生理活性物質生産2017

    • Author(s)
      南 博道
    • Organizer
      BioJapan2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 大腸菌を用いた単純な炭素源からのモルヒネ発酵生産系の構築2017

    • Author(s)
      中川 明、松村 栄太郎、小柳 喬、片山 高嶺、山本 憲二、佐藤 文彦、南 博道
    • Organizer
      日本分子生物学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物と微生物:イソキノリンアルカロイド生産の展望2016

    • Author(s)
      佐藤文彦
    • Organizer
      日本生物工学会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-29
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] イソキノリンアルカロイド生産制御系の構築に向けたCjWRKY1の機能展開 (ポスター)2016

    • Author(s)
      山田泰之、島田友恵、佐藤文彦
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      信州大学繊維学部
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] Total biosynthesis of opiates by engineered Escherichia coli.2016

    • Author(s)
      Nakagawa, A., Sato, F. and Minami, H.
    • Organizer
      Metabolic Engineering 11
    • Place of Presentation
      Awaji Yumebutai International Conference Center
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス3T3-L1 細胞を用いたイソキノリンアルカロイドの生理活性評価2016

    • Author(s)
      十亀真実、岩田優子、Chow Yit-lai、佐藤文彦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションホール、札幌
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] イソキノリンアルカロイド生合成系の発現制御に関わるCjWRKY1の翻訳後制御機構の解析2016

    • Author(s)
      山田泰之、佐藤文彦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションホール、札幌
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドラフトゲノムを用いたハナビシソウアルカロイド生合成に関わる新規P450の探索2016

    • Author(s)
      堀健太郎、山田泰之、佐藤文彦
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションホール
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Sphingobium sp. BD3100株のベルベリン脱メチレン化酵素の解析2016

    • Author(s)
      武田尚、石川和樹、若菜大悟、福田雅夫、佐藤文彦、細江智夫
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションホール、札幌
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸菌を利用したモルヒネの発酵生産2016

    • Author(s)
      中川 明、松村栄太郎、片山高嶺、小柳 喬、山本憲二、熊谷英彦、佐藤文彦、南 博道
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションホール、札幌
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization of plant functions using cultured plant cells, and biotechnological applications2016

    • Author(s)
      Sato F.
    • Organizer
      Science Conferences & Events Association (VISCEA) on “Plant Cells in Vitro: Theory and Practice”
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-02-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微生物発酵法による生薬生理活性物質生産と創薬展開2016

    • Author(s)
      南 博道
    • Organizer
      ライフサイエンス技術部会・反応分科会講演会「新世代微生物発酵法による有用物質生産の最前線 ~アルカロイドと抗生物質を中心に~」
    • Place of Presentation
      公益社団法人新化学技術推進協会、東京
    • Year and Date
      2016-02-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Lipid metabolism modulating activity of beznylisoquinolien alkaloids2015

    • Author(s)
      Chow, Y.-L., Iwata, Y., Sogame, M., Sato F.
    • Organizer
      Diabetes: New insights into molecular mechanisms and therapeutic strategies. Keystone Symposia on Molecular and Cellular Biology
    • Place of Presentation
      Westin Miyako Kyoto, Kyoto
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular characterization of O-methyltransferases involved in benzophenanthridine biosynthesis in Eschscholzia caflifornica2015

    • Author(s)
      Purwanto, Hori, K., Sato F.
    • Organizer
      Inaugural Symposium of the Phytochemical Society of Asia2015
    • Place of Presentation
      Tokushima Bunri Univ., Tokushima
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microbial production of benzylisoquinoline alkaloid 20152015

    • Author(s)
      Sato F. and Minami, H.
    • Organizer
      Inaugural Symposium of the Phytochemical Society of Asia2015
    • Place of Presentation
      Tokushima Bunri Univ., Tokushima
    • Year and Date
      2015-09-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ベルベリン生合成系を制御するCjWRKY1の翻訳後制御2015

    • Author(s)
      山田泰之、佐藤文彦
    • Organizer
      第33回日本植物細胞分子生物学会
    • Place of Presentation
      東京大学農学部、東京
    • Year and Date
      2015-08-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] CRISPR/Cas9 systemを用いたオウレンのゲノム編集2015

    • Author(s)
      西尾洋、佐藤文彦
    • Organizer
      第33回日本植物細胞分子生物学会
    • Place of Presentation
      東京大学農学部、東京
    • Year and Date
      2015-08-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] ドラフトゲノムを用いたハナビシソウアルカロイド生合成に関わる新規P450の探索2015

    • Author(s)
      堀健太郎、山田泰之、佐藤文彦
    • Organizer
      第33回日本植物細胞分子生物学会
    • Place of Presentation
      東京大学農学部、東京
    • Year and Date
      2015-08-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] Exploring novel P450s involved in isoquinoline alkaloid biosynthesis using draft genome sequence of Eschscholzia californica2015

    • Author(s)
      Hori, K., Yamada, Y., Sato F.
    • Organizer
      19th International Conference on Cytochrome P450 ; Biochemistry, Biophysics, and Biotechnology
    • Place of Presentation
      National Olympics Memorial Youth Center, Tokyo
    • Year and Date
      2015-06-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ドラフトゲノムデータを用いたハナビシソウアルカロイド生合成 P450 遺伝子の探索2015

    • Author(s)
      堀 健太郎、山田 泰之、佐藤 文彦
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山大学農学部(岡山)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] ベルベリン生合成系の転写因子 CjWRKY1 の翻訳後制御機構の解析2015

    • Author(s)
      山田 泰之、佐藤 文彦
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山大学農学部(岡山)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物発酵法による植物アルカロイド生産と生薬生理活性物質の創製2015

    • Author(s)
      南 博道
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山大学農学部(岡山)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 大腸菌を利用した医薬中間体候補化合物であるメチル化ノルラウダノソリンコレクションの作成2015

    • Author(s)
      中川明、杉本美菜世、松村栄太郎、小柳喬、片山高嶺、山本憲二、佐藤文彦、南博道
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山大学農学部(岡山)
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物を用いたイソキノリンアルカロイドstylopine生産系の構築2015

    • Author(s)
      岡野峻祐、堀健太郎、佐藤文彦
    • Organizer
      日本農芸化学会関西支部第488回講演会
    • Place of Presentation
      楽友会館(京都)
    • Year and Date
      2015-01-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] イソキノリンアルカロイド生合成マシナリー構築2014

    • Author(s)
      佐藤文彦
    • Organizer
      新学術領域研究 生合成マシナリー 第8回公開シンポジウム
    • Place of Presentation
      東京大学大学院薬学研究科総合研究棟薬学部講堂
    • Year and Date
      2014-12-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 微生物発酵法による植物アルカロイド生産2014

    • Author(s)
      南 博道
    • Organizer
      第32回バイオ技術シーズ公開会
    • Place of Presentation
      大阪
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] P450s and plant isoquinoline alkaloid biosynthesis2014

    • Author(s)
      Fumihiko Sato
    • Organizer
      12th International symposium on cytochrome P450: Biodiversity and biotechnology
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-09-24 – 2014-09-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Reconstruction of benzylisoquinoline alkaloid biosynthetic pathway in methylotrophic yeast with heterologous expression of cytochrome P450s2014

    • Author(s)
      K. Hori, S. Okano, F. Sato
    • Organizer
      12th International symposium on cytochrome P450: Biodiversity and biotechnology
    • Place of Presentation
      Kyoto
    • Year and Date
      2014-09-24 – 2014-09-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物発酵法による植物アルカロイド生産と創薬展開2014

    • Author(s)
      中川明、小柳喬、片山高嶺、山本憲二、佐藤文彦、熊谷英彦、南博道
    • Organizer
      第26回微生物シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2014-09-19 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物を用いたイソキノリンアルカロイド生合成経路の再構築2014

    • Author(s)
      堀健太郎、岡野峻祐、佐藤文彦
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      いわて県民情報交流センター(盛岡)
    • Year and Date
      2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] イソキノリンアルカロイド生合成系の転写因子CjWRKY1の転写後制御に関する解析2014

    • Author(s)
      山田泰之、佐藤文彦
    • Organizer
      第32回日本植物細胞分子生物学会(盛岡)大会・シンポジウム
    • Place of Presentation
      いわて県民情報交流センター(盛岡)
    • Year and Date
      2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物二次代謝産物、特にイソキノリンアルカロイドとその生理活性、2014

    • Author(s)
      佐藤文彦
    • Organizer
      京都大学生命科学研究科シンポジウム
    • Place of Presentation
      芝蘭会館(京都)
    • Year and Date
      2014-07-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] Plant Secondary Metabolism. In: eLS 20142014

    • Author(s)
      Sato, Fumihiko
    • Total Pages
      3
    • Publisher
      John Wiley & Sons Ltd
    • Related Report
      2014 Annual Research Report
  • [Remarks] ハナビシソウゲノムの解読から見えてきたイソキノリンアルカロイド生合成酵素遺伝子の進化

    • URL

      http://www.kyoto-u.ac.jp/ja/research/research_results/2017/171229_2.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 生命科学研究科全能性統御機構学分野ホームページ

    • URL

      http://www.callus.lif.kyoto-u.ac.jp/news/news-new.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 京都大学アカデミックデイ2017 植物は何故薬をつくるのだろう?

    • URL

      http://research.kyoto-u.ac.jp/academic-day/2017/fumihiko_sato/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 植物が有用物質生産を制限する仕組みとは -ベルベリン生合成を制 御する転写因子の働きを解明-

    • URL

      http://www.kyoto-u.ac.jp/ja/research/research_results/2016/160824_1.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 生命科学研究科全能性統御機構学分野 ニュース

    • URL

      http://www.callus.lif.kyoto-u.ac.jp

    • Related Report
      2016 Annual Research Report
  • [Remarks] 全能性統御機構学分野

    • URL

      http://www.lif.kyoto-u.ac.jp/labs/callus/

    • Related Report
      2015 Annual Research Report
  • [Remarks] モルフィナンアルカロイド生産のための微生物プラットホームの確立

    • URL

      http://www.kyoto-u.ac.jp/ja/research/research_results/2015/160205_1.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] ゲノムの中から薬づくりの道具を探す

    • URL

      http://research.kyoto-u.ac.jp/academic-day/2015/22/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学アカデミックデイ 植物の薬用成分を効率的に作る

    • URL

      http://research.kyoto-u.ac.jp/academic-day/2014/07/

    • Related Report
      2014 Annual Research Report

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Published: 2014-06-05   Modified: 2019-05-15  

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