|Budget Amount *help
¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
|Outline of Final Research Achievements
In mammalian females one of two X chromosomes is inactivated for gene dosage compensation, and this phenomenon is called X chromosome inactivation or XCI. Timing of X chromosome reactivation and inactivation during embryonic development remains elusive. In this study, we developed 3D whole-mount RNA-FISH method as well as Xvis mouse strain, in which XCI status can be visualized, and applied these techniques to XCI studies. Using these novel method and resources, we succeeded to determine timing of XCI reversal and initiation of random XCI during peri-implantation development for the first time. Also, by conducting image analyses of tissues/organs of Xvis mice, we could clearly demonstrate the presence of genetic mosaicism in mammalian females.