• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Lignification in polysaccharide matrix mimicking wood cell wall and clarification of lignin structure -control factor

Research Project

Project/Area Number 26252022
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionHokkaido University

Principal Investigator

Uraki Yasumitsu  北海道大学, 農学研究院, 教授 (90193961)

Co-Investigator(Kenkyū-buntansha) 重冨 顕吾  北海道大学, 農学研究院, 講師 (20547202)
玉井 裕  北海道大学, 農学研究院, 准教授 (50281796)
吉永 新  京都大学, 農学研究科, 准教授 (60273489)
岸本 崇生  富山県立大学, 工学部, 准教授 (60312394)
高部 圭司  京都大学, 農学研究科, 教授 (70183449)
幸田 圭一  北海道大学, 農学研究院, 講師 (80322840)
Project Period (FY) 2014-06-27 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥40,950,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥9,450,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2014: ¥21,840,000 (Direct Cost: ¥16,800,000、Indirect Cost: ¥5,040,000)
Keywordsリグニン / ヘミセルロース / 多糖類マトリックス / 脱水素重合 / 分枝構造 / グルコマンナン / キシラン / 脱水素重合物 / バイオマス / 木化 / リグニンの溶液物性 / モノリグノールの移動 / 細胞壁模倣多糖類マトリックス / 免疫標識 / 電界放射型透過型電子顕微鏡 / 免疫蛍光標識 / 同位体顕微鏡
Outline of Final Research Achievements

An aim of this study is to elucidate lignin formation mechanism (lignification), especially influence of hemicelloses, with polysaccharide matrix mimicking wood cell wall structure. Polysaccharide matrix was successfully prepared by the deposition of hemicelluloses, xylan and glucomannan, onto bacterial cellulose. Dehydrogenative polymerization of coniferyl alcohol, which was one of monolignols, was attempted in the presence of such polysaccharide matrix. As a result, the polymerization was accelerated by xylan deposition, but was inhibited by glucomannan.
In addition, the structure of the resultant polymerized product (Dehydronation Polymer: DHP) was investigated on a size-exclusion chromatography equipped with a laser light-scattering detector by the comparison with solution structure of a non-branched lignin model compound that is comprised of only 8-O-4’ linkage. This investigation revealed that DHP had a significant frequency of branched structure.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (24 results)

All 2018 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (21 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Journal Article] Heterogeneous distribution of xylan and lignin in tension wood G-layers of the S1+G type in several Japanese hardwood2017

    • Author(s)
      Higaki A, Yoshinaga A, Takabe K
    • Journal Title

      Tree Physiology

      Volume: 37 Issue: 12 Pages: 1767-1775

    • DOI

      10.1093/treephys/tpx144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of amphipathic lignin derivatives with cellobiohydrolase groups improves enzymatic saccharification of lignocellulosics.2017

    • Author(s)
      Y. Yamamoto, N. Cheng, K. Koda, K. Igarashi, Y. Tamai and Y. Uraki
    • Journal Title

      Cellulose

      Volume: 24 Issue: 2 Pages: 1849-1862

    • DOI

      10.1007/s00248-017-0952-8

    • NAID

      120006415998

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dehydrogenative polymerization of coniferyl alcohol in artificial polysaccharides matrices: Effects of xylan on the polymerization2015

    • Author(s)
      Q. Li, K. Koda, A. Yoshinaga, K. Takabe, M. Shimomura, Y. Hirai, Y. Tamai, Y. Uraki
    • Journal Title

      Journal of Agricultural and Food Chemistry,

      Volume: 印刷中 Issue: 18 Pages: 4613-4620

    • DOI

      10.1021/acs.jafc.5b01070

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] S1+G型の引張あて材木部繊維におけるキシランとリグニンの分布2018

    • Author(s)
      吉永 新、 檜垣 綾乃、高部圭司
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 偏光顕微ラマン分光法によるミズナラ材細胞壁におけるリグニン芳香核の配向解析2018

    • Author(s)
      有泉 慧、吉永 新、高部 圭司
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] モノクローナル抗体に夜シラカシ正常材及び引張あて材におけるリグニンの免疫局在2018

    • Author(s)
      中村 雄太、吉永 新、高部 圭司
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コニフェリルアルコールの脱水素重合に及ぼすヘミセルロースの効果2018

    • Author(s)
      松尾朱美、古川貴大、玉井裕、幸田圭一、浦木康光、吉永新、高部圭司
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Elucidation of hemicellulose functions on lignification using artificial wood cell wall based on honeycomb-patterned cellulose2018

    • Author(s)
      Yasumitsu Uraki
    • Organizer
      255th ACS National Meeting & Exposition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 樹木組織中での安定同位体標識モノリグノール類の移動 ~同位体顕微鏡による可視化~2017

    • Author(s)
      小川真由、幸田圭一、重冨顕吾、坂本直哉、高部圭司、吉永 新、浦木康光
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      九州大学箱崎キャンパス
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 偏光顕微ラマン分光法によるスギ仮道管壁におけるリグニンの配向解析2017

    • Author(s)
      有泉 慧、吉永 新、高部圭司
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      九州大学箱崎キャンパス
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒノキの形成中仮道管壁内表面におけるセルロースとヘミセルロースの分子配置Ⅰ ーヘミセルラーゼ処理による構造変化からの考察ー2017

    • Author(s)
      櫻井みづき、高部圭司
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      アクロス福岡(福岡)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Orientation of lignin in tracheid secondary walls in Cryptomeria japonica as revealed by polarized Raman microscopy2017

    • Author(s)
      Ariizumi, K, Yoshinaga, A, Takabe, K
    • Organizer
      The 9th Pacific Regional Wood Anatomy Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Function of glucomannan in lignin formation using artificial wood cell wall. Proc. 19th International Symposium on Wood, Fiber and Pulping Chemistry2017

    • Author(s)
      Takahiro Furukawa, Arata Yoshinaga, Keiji Takabe, Yutaka Tamai, Keiichi Koda, Yasumitsu Uraki
    • Organizer
      Proc. 19th International Symposium on Wood, Fiber and Pulping Chemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization of the distribution of stable isotope labelled-monolignols in woody tissue by isotope microscopic observation2017

    • Author(s)
      Keiichi Koda, Mayu Ogawa, Kengo Shigetomi, Naoya Sakamoto, Arata Yoshinaga, Keiji Takabe, Yasumitsu Uraki
    • Organizer
      Proc. 19th International Symposium on Wood, Fiber and Pulping Chemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リグニンアナログの溶液中における構造2016

    • Author(s)
      重富顕吾、山本陽子、幸田圭一、岸本崇生、浦木康光
    • Organizer
      リグニン討論会
    • Place of Presentation
      京都大学維持キャンパス
    • Year and Date
      2016-10-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Monoclonal antibody directed against 8-O-4' linked structure of lignin.2016

    • Author(s)
      Yoshinaga A, Wada M, Hoshikawa S, Kiyoto S, Kamitakahara H, Takabe K:
    • Organizer
      The XIVth Cell Wall Meeting
    • Place of Presentation
      Chania, Greece
    • Year and Date
      2016-06-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 同位体顕微鏡を用いたモノリグノール結合セルロースフィルムの観察2016

    • Author(s)
      小川真由、幸田圭一、浦木康光
    • Organizer
      日本木材学会
    • Place of Presentation
      名古屋大学(愛知県、名古屋市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] G層に存在するペルオキシダーゼを用いた人為的木化2016

    • Author(s)
      辻井珠奈,吉永 新,高部圭司
    • Organizer
      第66回日本木材学会大会
    • Place of Presentation
      名古屋大学(愛知県、名古屋市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] モノクローナル抗体を用いたリグニン中の8-O-4′型構造の標識2016

    • Author(s)
      星川慎一郎,吉永 新,高部圭司
    • Organizer
      第66回日本木材学会大会
    • Place of Presentation
      名古屋大学(愛知県、名古屋市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 免疫標識と組織化学染色による、ミズメ木部における木化および8-5′型構造の形成過程の解析2016

    • Author(s)
      清都晋吾,吉永 新,高部圭司
    • Organizer
      第66回日本木材学会大会
    • Place of Presentation
      名古屋大学(愛知県、名古屋市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Thermal molding of lignin based on its fusibility2015

    • Author(s)
      Yasumitsu Uraki, Xiangyu You, Keiichi Koda, Satoshi Kubo, Tatsuhiko Yamada
    • Organizer
      18th International Symposium on Wood, Fiber and PulpChemistry
    • Place of Presentation
      Vienna, Anstria
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Honeycomb-patterned cellulose films as a promising tool to investigate deformation of wood cross section and wood cell wall formation.2015

    • Author(s)
      Yasumitsu Uraki, Qiang Li, Teuku Beruna Bardant, Keiichi Koda
    • Organizer
      249th ACS National Meeting & Exposition.
    • Place of Presentation
      Denver, USA
    • Year and Date
      2015-03-22 – 2015-03-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Xylan as a schaffold for DHP deposition on cellulose2015

    • Author(s)
      Li Qiang, Tasaki Yuka, Yoshinaga Arata, Takabe Keiji, Koda Keiichi and Uraki Yasumitsu
    • Organizer
      International Symposium on Wood Science and Technology 2015
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-03-15 – 2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] DHP formation in the matrix of hemicellulose-deposited honeycomb-patterned cellulose.2014

    • Author(s)
      Qiang Li, Keiichi Koda, Yasumitsu Uraki
    • Organizer
      XXVIIth International Conference on Polyphenols & 8th Tannnin Conference
    • Place of Presentation
      Nagoya Univ.. Japan
    • Year and Date
      2014-09-02 – 2014-09-06
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-11-20   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi