| Budget Amount *help |
¥39,780,000 (Direct Cost: ¥30,600,000、Indirect Cost: ¥9,180,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2014: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we aimed at better understanding of ion-transporting mechanisms in gill ionocytes, and profiles of K+ and Cs+ metabolisms in fish. The following are the main findings:
1) We identified 6 isoforms of Na+/K+-ATPases in tilapia gills. 2) Foxi3 functions as a transcription factor essential for differentiation induction and proliferation of ionocytes in tilapia. 3) We identified full-length sequences of renal outer medullary potassium channel (ROMK) cDNAs from four salmonid fishes. Their deduced amino acid sequences showed 97 to 100% homology. 4) Tilapia prolactin is important in maintaining ionocytes in the gills, and cortisol synergistically enhances the ionocyte-maintaining effect of prolactin. 5) In the gastrointestinal tract of tilapia, K+ absorption takes place in the stomach through HKA and KCNQ1, and in the intestine through NKCC2 and NCCb. These findings contribute to establishment of decontamination techniques of radioactive cesium in fishes.
|
