Prevention of refractory diseases in animals by novel strategies based on replication-incompetent recombinant viruses
| Project/Area Number |
26252048
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HORIMOTO TAISUKE 東京大学, 大学院農学生命科学研究科(農学部), 教授 (00222282)
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| Co-Investigator(Kenkyū-buntansha) |
村上 晋 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10636757)
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| Project Period (FY) |
2014-04-22 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥35,100,000 (Direct Cost: ¥27,000,000、Indirect Cost: ¥8,100,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2014: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Keywords | ウイルス / 感染症 / 獣医学 / ワクチン / インフルエンザ / 腫瘍 |
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| Outline of Final Research Achievements |
This study includes (1) “Generation of replication-incompetent virus vaccines against influenza”, and (2) “Anti-tumor therapy using oncolytic adenoviruses in dogs. First, we successfully established MDCK cells stably expressing each HA (H1-H15), which were required to generate replication-incompetent recombinant viruses with each HA. Actually, we generated a couple of such viruses and found their immunogenicity. Second, we established adenovirus reverse genetics system based on bacterial artificial chromosome and successfully generated a bat adenovirus-recombinant virus with E1A/B deletion to assess its anti-tumor effect as an oncolytic adenovirus, which targeted dog tumors.
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| Academic Significance and Societal Importance of the Research Achievements |
鳥インフルエンザを防御するための1回感染型非増殖性ウイルスの構築、さらにp10分子等を同時に発現するワクチン候補ウイルスの構築は、本研究グループ以外では例がない。複数のHA亜型に対応できる多価ワクチンの構築は、新興性インフルエンザに対応できる独創的な戦略であり、本研究は今後の展開に貢献する。一方、腫瘍細胞特異的に増殖する非病原性アデノウイルスを構築し、犬の抗ガン療法としての実用化を目指すのは国内では初めての戦略である。。今回明らかになったコウモリアデノウイルスをベースとする腫瘍溶解性ウイルスの応用性は今後の研究に貢献する。
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Influenza A virus infection in Japanese wild boars (<i>Sus scrofa leucomystax</i>)2017
Author(s)
Shimoda H, Nguyen DV, Yonemitsu K, Minami S, Nagata N, Hara N, Kuwata R, Murakami S, Kodera Y, Takeda T, Yoshikawa Y, Horimoto T, Maeda K.
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Journal Title
J. Vet. Med. Sci.
Volume: 印刷中
Issue: 5
Pages: 848-851
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Nationwide distribution of bovine influenza D virus infection in Japan.2016
Author(s)
Horimoto T, Hiono T, Mekata H, Odagiri T, Lei Z, Kobayashi T, Norimine J, Inoshima Y, Hikono H, Murakami K, Sato R, Murakami H, Sakaguchi M, Ishii K, Ando T, Otomaru K, Ozawa M, Sakoda Y, Murakami S.
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Journal Title
Plos one
Volume: 11 (9)
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Akabane virus nonstructural protein NSm regulates viral growth and pathogenicity in a mouse model.2016
Author(s)
Ishihara Y, Shioda C, Bangphoomi N, Sugiura K, Saeki K, Tsuda S, Iwanaga T, Takenaka-Uema A, Kato K, Murakami S, Udhida K, Akashi H, Horimoto T.
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Journal Title
J Vet Med Sci
Volume: 79 (9)
Pages: 1391-1397
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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