• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Prevention of refractory diseases in animals by novel strategies based on replication-incompetent recombinant viruses

Research Project

Project/Area Number 26252048
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Veterinary medical science
Research InstitutionThe University of Tokyo

Principal Investigator

HORIMOTO TAISUKE  東京大学, 大学院農学生命科学研究科(農学部), 教授 (00222282)

Co-Investigator(Kenkyū-buntansha) 村上 晋  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10636757)
Project Period (FY) 2014-04-22 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥35,100,000 (Direct Cost: ¥27,000,000、Indirect Cost: ¥8,100,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2014: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Keywordsウイルス / 感染症 / 獣医学 / ワクチン / インフルエンザ / 腫瘍
Outline of Final Research Achievements

This study includes (1) “Generation of replication-incompetent virus vaccines against influenza”, and (2) “Anti-tumor therapy using oncolytic adenoviruses in dogs. First, we successfully established MDCK cells stably expressing each HA (H1-H15), which were required to generate replication-incompetent recombinant viruses with each HA. Actually, we generated a couple of such viruses and found their immunogenicity. Second, we established adenovirus reverse genetics system based on bacterial artificial chromosome and successfully generated a bat adenovirus-recombinant virus with E1A/B deletion to assess its anti-tumor effect as an oncolytic adenovirus, which targeted dog tumors.

Academic Significance and Societal Importance of the Research Achievements

鳥インフルエンザを防御するための1回感染型非増殖性ウイルスの構築、さらにp10分子等を同時に発現するワクチン候補ウイルスの構築は、本研究グループ以外では例がない。複数のHA亜型に対応できる多価ワクチンの構築は、新興性インフルエンザに対応できる独創的な戦略であり、本研究は今後の展開に貢献する。一方、腫瘍細胞特異的に増殖する非病原性アデノウイルスを構築し、犬の抗ガン療法としての実用化を目指すのは国内では初めての戦略である。。今回明らかになったコウモリアデノウイルスをベースとする腫瘍溶解性ウイルスの応用性は今後の研究に貢献する。

Report

(5 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(29 results)

All 2018 2017 2016 2015 2014

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 3 results,  Acknowledgement Compliant: 10 results) Presentation (18 results)

  • [Journal Article] Influenza C and D Viruses Package Eight Organized Ribonucleoprotein Complexes2018

    • Author(s)
      Nakatsu Sumiho、Murakami Shin、Shindo Keiko、Horimoto Taisuke、Sagara Hiroshi、Noda Takeshi、Kawaoka Yoshihiro
    • Journal Title

      J Virol

      Volume: 92

    • DOI

      10.1128/jvi.02084-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines.2017

    • Author(s)
      Taniguchi S, Maeda K, Horimoto T, Masangkay JS, Puentespina Jr R., Eres E, et al.
    • Journal Title

      Archives of Virology

      Volume: - Pages: 1529-1539

    • DOI

      10.1007/s00705-017-3251-2

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Influenza A virus infection in Japanese wild boars (<i>Sus scrofa leucomystax</i>)2017

    • Author(s)
      Shimoda H, Nguyen DV, Yonemitsu K, Minami S, Nagata N, Hara N, Kuwata R, Murakami S, Kodera Y, Takeda T, Yoshikawa Y, Horimoto T, Maeda K.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 印刷中 Issue: 5 Pages: 848-851

    • DOI

      10.1292/jvms.17-0052

    • NAID

      130005635451

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nationwide distribution of bovine influenza D virus infection in Japan.2016

    • Author(s)
      Horimoto T, Hiono T, Mekata H, Odagiri T, Lei Z, Kobayashi T, Norimine J, Inoshima Y, Hikono H, Murakami K, Sato R, Murakami H, Sakaguchi M, Ishii K, Ando T, Otomaru K, Ozawa M, Sakoda Y, Murakami S.
    • Journal Title

      Plos one

      Volume: 11 (9) Pages: 1-7

    • DOI

      10.1371/journal.pone.0163828

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Akabane virus nonstructural protein NSm regulates viral growth and pathogenicity in a mouse model.2016

    • Author(s)
      Ishihara Y, Shioda C, Bangphoomi N, Sugiura K, Saeki K, Tsuda S, Iwanaga T, Takenaka-Uema A, Kato K, Murakami S, Udhida K, Akashi H, Horimoto T.
    • Journal Title

      J Vet Med Sci

      Volume: 79 (9) Pages: 1391-1397

    • NAID

      130005598014

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Influenza D virus infection in herd of cattle, Japan.2016

    • Author(s)
      Murakami S, Endoh M, Kobayashi T, Takenaka-Uema A, Chambers JK, Uchida K, Nishihara M, Hause B, Horimoto T.
    • Journal Title

      Emerg Infect Dis

      Volume: 22 (8) Pages: 1517-1519

    • DOI

      10.3201/eid2208.160362

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of a recombinant Akabane mutant virus with knockout of a nonstructural protein NSs in a pregnant goat model.2016

    • Author(s)
      Takenaka-Uema A, Horimoto T, et al.
    • Journal Title

      Virol Sin

      Volume: 印刷中 Pages: 274-277

    • DOI

      10.1007/s12250-015-3704-2

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of an improved reverse genetics system for Akabane bunyavirus.2016

    • Author(s)
      Takenaka-Uema A, Horimoto T, et al.
    • Journal Title

      J Virol Methods

      Volume: 232 Pages: 16-20

    • DOI

      10.1016/j.jviromet.2015.12.014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Generation of a recombinant Akabane virus expressing enhanced green fluorescent protein.2015

    • Author(s)
      Takenaka-Uema A, Horimoto T, et al.
    • Journal Title

      J. Virol.

      Volume: 189 Pages: 9477-9484

    • DOI

      10.1128/jvi.00681-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A bivalent vaccine based on a replication-incompetent influenza virus protects against Streptococcus pneumoniae and influenza virus infection.2014

    • Author(s)
      Katsura H, Piao Z, Iwatsuki-Horimoto K, Akeda Y, Watanabe S, Horimoto T, Oishi K, Kawaoka Y.
    • Journal Title

      J. Virol.

      Volume: 88 Pages: 13410-13417

    • DOI

      10.1128/jvi.01205-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Serological evidence of infection of dogs with human influenza viruses in Japan2014

    • Author(s)
      Horimoto T, Gen F, Murakami S, Iwatsuki-Horimoto K, Kato K, Akashi H, Hisasue M, Sakaguchi M, Kawaoka Y, Maeda K
    • Journal Title

      Vet. Rec.

      Volume: 174 Pages: 96-96

    • DOI

      10.1136/vr.101929

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 国内のコウモリから分離された2種類のアデノウイルスの性状解析2017

    • Author(s)
      小林知也、丸山隼輝、松郷宙倫、前田健、高田礼人、村上晋、堀本泰介
    • Organizer
      第159回日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 鳥インフルエンザウイルス弱毒株の哺乳類細胞における増殖性を上げる変異2017

    • Author(s)
      松郷宙倫、堀本泰介
    • Organizer
      第1回獣医RNAウイルス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] H9N2インフルエンザウイルスが哺乳類へ馴化するために必要なゲノム変異の探索2017

    • Author(s)
      神木春彦、堀本泰介
    • Organizer
      第1回獣医RNAウイルス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 国内のコウモリから分離された2種類のアデノウイルスの性状解析2017

    • Author(s)
      小林知也、丸山隼輝、松郷宙倫、前田健、高田礼人、村上晋、堀本泰介
    • Organizer
      第65回日本ウイルス学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Serological survey reveals the wide distribution of bovine influenza D virus infection in Japan.2016

    • Author(s)
      Horimoto T, Odagiri T, Lei Z, Kobayashi T, Uema A, Mekata H, Norimine J, Hikono H, et al.
    • Organizer
      第64回日本ウイルス学会
    • Place of Presentation
      北海道札幌市(札幌コンベンションセンター)
    • Year and Date
      2016-10-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of bovine influenza D virus isolated in Japan.2016

    • Author(s)
      Murakami S, Kobayashi T, Sato T, Horimoto T
    • Organizer
      第64回日本ウイルス学会
    • Place of Presentation
      北海道札幌市(札幌コンベンションセンター)
    • Year and Date
      2016-10-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 国内のモモジロコウモリ(Myotis macrodactylus)から分離された新規アデノウイルス2016

    • Author(s)
      小林知也、村上晋、松郷宙倫、神木春彦、前田健、堀本泰介
    • Organizer
      第159回日本獣医学会
    • Place of Presentation
      神奈川県藤沢市(日本大学)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 鳥インフルエンザウイルス弱毒株の哺乳類細胞における増殖性に関わる変異2016

    • Author(s)
      松郷宙倫、村上晋、堀本泰介
    • Organizer
      第159回日本獣医学会
    • Place of Presentation
      神奈川県藤沢市(日本大学)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 豚サーコウイルスゲノムを基盤とするエピゾーマルベクターの検討2015

    • Author(s)
      小林知也、堀本泰介ら
    • Organizer
      第63回日本ウイルス学会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 外来遺伝子高発現組換えアカバネウイルスの作製2015

    • Author(s)
      上間亜希子、堀本泰介ら
    • Organizer
      第63回日本ウイルス学会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 豚サーコウイルスゲノムを基盤とするエピゾーマルベクターの検討2015

    • Author(s)
      小林知也、堀本泰介ら
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学(十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] H9N2インフルエンザウイルスが哺乳類へ馴化するために必要なゲノム変異の探索2015

    • Author(s)
      神木春彦、堀本泰介ら
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学(十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] フィリピンのコウモリから分離されたプテロパインオルソレオウイルスの性状解析及び疫学的研究2015

    • Author(s)
      谷口怜、堀本泰介ら
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学(十和田)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] GFP発現組換えアカバネウイルスの作出2014

    • Author(s)
      上間亜希子、堀本泰介ら
    • Organizer
      第62回日本ウイルス学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] A型インフルエンザウイルス(H1N1)の鶏卵における高増殖性決定因子の解析2014

    • Author(s)
      飯村太朗、堀本泰介ら
    • Organizer
      第62回日本ウイルス学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 野生動物に対するA型インフルエンザウイルス抗体検出系の検討2014

    • Author(s)
      奥村実紀、堀本泰介ら
    • Organizer
      第62回日本ウイルス学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] A型インフルエンザウイルス(H1N1)の鶏卵における高増殖性決定因子の解析2014

    • Author(s)
      飯村太朗、堀本泰介ら
    • Organizer
      第157回日本獣医学会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 野生動物に対するA型インフルエンザウイルス抗体検出系の検討2014

    • Author(s)
      奥村実紀、堀本泰介ら
    • Organizer
      第157回日本獣医学会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-24   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi