Molecular basis of formation and regulation of insect holocentric chromosome
Project/Area Number |
26252057
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Insect science
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Research Institution | Kyushu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
佐藤 昌直 北海道大学, 農学研究院, 助教 (20517693)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2016: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
|
Keywords | 分散型動原体 / 染色体 / カイコ / 遺伝子ネットワーク / クロマチン構造 / クロマチン / エピジェネティクス / 分散型動原体染色体 / 複製 / 昆虫 / 動原体 |
Outline of Final Research Achievements |
Lepidopteran insects are known to have specific chromosomes called heliocentric chromosome having multiple centromeres along the entire length. In this study, we clarified the molecular basis of kinetochore formation of the heliocentric chromosome and newly found six centromere-related genes specific to silkworm using the gene network analysis. Based on these results and analysis on replication related genes, an artificial chromosome functioning by tethering DNA replication factor and kinetochore protein was synthesized. On the other hand, as for the genome-wide gene expression regulation and molecular basis of chromatin structure, we focused on histone acetylation and deacetylation enzymes, and performed gene knockdown on these genes. As a result, we found that HDAC 8 plays an important role in the silkworm.
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Report
(5 results)
Research Products
(16 results)
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[Presentation] Characterization of Histone H1 Variants in Bombyx mori2017
Author(s)
Takumi Yano, Yoshiki Morifuji, Masato Hino, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe
Organizer
The 5th Asia-Pacific Congress of Sericulture and Insect Biotechnology
Place of Presentation
Bangkok, Thailand
Year and Date
2017-02-28 – 2017-03-02
Related Report
Int'l Joint Research
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[Presentation] Characterization of silkworm replication related proteins for making an artificial chromosome2015
Author(s)
Masato Hino, Hiroaki Mon, Li Zhu, Mami Yamashita, Kazuma Hirata, Dan Zheng, Jian Xu, Ming-Ming Ji, Daisuke Morokuma, JaeMan Lee, Takahiro Kusakabe
Organizer
The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology
Place of Presentation
Busan, Korea
Year and Date
2015-04-22 – 2015-04-24
Related Report
Int'l Joint Research