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Discovery of novel blood-stage malaria vaccine candidates based on the molecular function

Research Project

Project/Area Number 26253026
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionEhime University

Principal Investigator

TSUBOI Takafumi  愛媛大学, プロテオサイエンスセンター, 教授 (00188616)

Co-Investigator(Kenkyū-buntansha) 高島 英造  愛媛大学, プロテオサイエンスセンター, 准教授 (50366762)
Research Collaborator MORITA Masayuki  愛媛大学, プロテオサイエンスセンター, 研究員 (60709632)
Project Period (FY) 2014-06-27 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2016: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2015: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2014: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Keywordsマラリア / 赤血球期ワクチン / コムギ無細胞法 / コンビネーションワクチン
Outline of Final Research Achievements

In order to identify novel blood-stage malaria vaccine candidates of Plasmodium falciparum, we have expressed 142 putative merozoite specific recombinant proteins. Among them, 34 bounded to the RBC surface. We then analyzed extent of polymorphism in four promising RBC-binding proteins, PfRipr, PfRALP1, PfGAMA, and PfMSPDBL1 using clinical isolates from a region of high malaria transmission in Uganda. PfRipr gene was most highly conserved among the four genes investigated. In addition, we expressed recombinant proteins for the four candidates based on P. falciparum laboratory strain 3D7 sequences, immunized rabbits to obtain specific antibodies and performed functional growth inhibition assay (GIA) on the heterologous FVO parasite strain. The GIA activity of anti-PfRipr antibody was highest among the four antibodies even on the polymorphic FVO parasite strain. Thus, PfRipr is regarded as a promising blood-stage malaria vaccine against P. falciparum.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (37 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (12 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 12 results,  Open Access: 6 results,  Acknowledgement Compliant: 9 results) Presentation (19 results) (of which Int'l Joint Research: 7 results,  Invited: 13 results) Remarks (2 results)

  • [Int'l Joint Research] Burnet institute/Walter & Elisa Hall Institute(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Med Biotech Laboratories(ウガンダ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Mahidol University(Thailand)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Kangwon National University(韓国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes.2016

    • Author(s)
      Bargieri DY, Thiberge S, Tay CL, Carey AF, Rantz A, Hischen F, Lorthiois A, Straschil U, Singh P, Singh S, Triglia T, Tsuboi T, Cowman A, Chitnis C, Alano P, Baum J, Pradel G, Lavazec C, Menard R.
    • Journal Title

      Cell Host Microbe.

      Volume: 20 Issue: 5 Pages: 618-630

    • DOI

      10.1016/j.chom.2016.10.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of Plasmodium falciparum reticulocyte binding protein homologue 5-interacting protein, PfRipr, as a highly conserved blood-stage malaria vaccine candidate.2016

    • Author(s)
      Ntege EH, Arisue N, Ito D, Hasegawa T, Palacpac NM, Egwang TG, Horii T, Takashima E, Tsuboi T.
    • Journal Title

      Vaccine

      Volume: 34 Issue: 46 Pages: 5612-5622

    • DOI

      10.1016/j.vaccine.2016.09.028

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA) binds human erythrocytes independent of Duffy antigen status.2016

    • Author(s)
      Cheng Y, Lu F, Wang B, Li J, Han JH, Ito D, Kong DH, Jiang L, Wu J, Ha KS, Takashima E, Sattabongkot J, Cao J, Nyunt MH, Kyaw MP, Desai SA, Miller LH, Tsuboi T, Han ET.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 35581-35581

    • DOI

      10.1038/srep35581

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The association between naturally acquired IgG subclass specific antibodies to the PfRH5 invasion complex and protection from Plasmodium falciparum malaria.2016

    • Author(s)
      Weaver R, Reiling L, Feng G, Drew DR, Mueller I, Siba PM, Tsuboi T, Richards JS, Fowkes FJ, Beeson JG.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 33094-33094

    • DOI

      10.1038/srep33094

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Antibody responses to Plasmodium falciparum and Plasmodium vivax blood-stage and sporozoite antigens in the postpartum period.2016

    • Author(s)
      McLean AR, Boel ME, McGready R, Ataide R, Drew D, Tsuboi T, Beeson JG, Nosten F, Simpson JA, Fowkes FJ.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 32159-32159

    • DOI

      10.1038/srep32159

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plasmodium vivax gametocyte proteins, Pvs48/45 and Pvs47, induce transmission-reducing antibodies by DNA immunization2015

    • Author(s)
      Tachibana M, Suwanabun N, Kaneko O, Iriko H, Otsuki H, Sattabongkot J, Kaneko A, Herrera S, Torii M, Tsuboi T
    • Journal Title

      Vaccine

      Volume: 33 Issue: 16 Pages: 1901-1908

    • DOI

      10.1016/j.vaccine.2015.03.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Rodent malaria lactate dehydrogenase assay provides a high throughput solution for in vivo vaccine studies2015

    • Author(s)
      Otsuki H, Yokouchi Y, Iyoku N, Tachibana M, Tsuboi T, Torii M
    • Journal Title

      Parasitol Int

      Volume: 64 Issue: 4 Pages: 60-63

    • DOI

      10.1016/j.parint.2015.02.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immunoprofiling of the tryptophan-rich antigen family in Plasmodium vivax2015

    • Author(s)
      Wang B, Lu F, Cheng Y, Chen JH, Jeon HY, Ha KS, Cao J, Nyunt MH, Han JH, Lee SK, Kyaw MP, Sattabongkot J, Takashima E, Tsuboi T, Han ET
    • Journal Title

      Infect Immun

      Volume: 83 Issue: 8 Pages: 3083-3095

    • DOI

      10.1128/iai.03067-14

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Application of wheat germ cell-free protein expression system for novel malaria vaccine candidate discovery2014

    • Author(s)
      Arumugam TU, Ito D, Takashima E, Tachibana M, Ishino T, Torii M, Tsuboi T
    • Journal Title

      Expert Review of Vaccines

      Volume: 13 Issue: 1 Pages: 75-85

    • DOI

      10.1586/14760584.2014.861747

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Tricomponent complex loaded with a mosquito-stage antigen of the malaria parasite induces potent transmission-blocking immunity2014

    • Author(s)
      Arakawa T, Tsuboi T, Sattabongkot J, Sakao K, Torii M, Miyata T
    • Journal Title

      Clinical and Vaccine Immunology

      Volume: 21 Issue: 4 Pages: 561-569

    • DOI

      10.1128/cvi.00053-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Profiling the humoral immune responses to Plasmodium vivax infection and identification of candidate immunogenic rhoptry-associated membrane antigen (RAMA)2014

    • Author(s)
      Lu F, Li J, Wang B, Cheng Y, Kong DH, Cui L, Ha KS, Sattabongkot J, Tsuboi T, Han ET
    • Journal Title

      Journal of Proteomics

      Volume: 102 Pages: 66-82

    • DOI

      10.1016/j.jprot.2014.02.029

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immunogenicity and antigenicity of Plasmodium vivax merozoite surface protein 102014

    • Author(s)
      Cheng Y, Wang B, Sattabongkot J, Lim CS, Tsuboi T, Han ET
    • Journal Title

      Parasitology Research

      Volume: 113 Issue: 7 Pages: 2559-2568

    • DOI

      10.1007/s00436-014-3907-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Post-genome malaria vaccine research.2017

    • Author(s)
      Tsuboi T
    • Organizer
      Japan-Brazil Malaria Research Workshop: fostering new partnerships
    • Place of Presentation
      Sao Paulo, Brazil
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Post-genome malaria vaccine candidate discovery by wheat germ cell-free system2016

    • Author(s)
      Tsuboi T
    • Organizer
      Joint International Tropical Medicine Meeting 2016
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2016-12-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ポストゲノム生化学研究:マラリアワクチン研究の突破口2016

    • Author(s)
      坪井敬文、高島英造、森田将之
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター、宮城県仙台市
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Reverse vaccinology approach: Postgenome malaria blood-stage vaccine candidate discovery.2016

    • Author(s)
      Tsuboi T, Ntege EH, Takashima E, Morita M, Ito D, Cowman A.
    • Organizer
      International Congress for Tropical Medicine and Malaria 2016
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      2016-09-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Approaches to malaria vaccine development.2016

    • Author(s)
      Tsuboi T
    • Organizer
      Workshop on Tools for Malaria Eradication
    • Place of Presentation
      RIKEN Yokohama Campus, Yokohama, Kanagawa, Japan
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ポストゲノムマラリアワクチン研究は宿主寄生体関係の総体的理解につながる2015

    • Author(s)
      坪井敬文
    • Organizer
      第88回日本生化学会大会・第38回日本分子生物学会年会 合同大会
    • Place of Presentation
      神戸ポートアイランド、神戸市、兵庫県
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Pre-erythrocytic vaccines for malaria elimination2015

    • Author(s)
      Tsuboi T
    • Organizer
      Malaria R&D in a Time of Global Partnerships
    • Place of Presentation
      東京大学、東京都
    • Year and Date
      2015-06-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] an integrated approach to tackling malaria in Uganda with special reference to novel malaria vaccine candidate discovery2015

    • Author(s)
      Tsuboi T, Kanoi BN, Egwang TG, Horii T
    • Organizer
      E-JUST 2nd International Conference on Innovative Engineering
    • Place of Presentation
      Bibliotheca Alexandria, Alexandria, Egypt
    • Year and Date
      2015-05-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] WGCFS: an innovative technology for post-genome malaria vaccine research2015

    • Author(s)
      Tsuboi T
    • Organizer
      25th Annual Molecular Parasitology/Vector Biology Symposium
    • Place of Presentation
      University of Georgia, Athens, USA
    • Year and Date
      2015-04-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Wheat germ cell-free protein synthesis system (WGCFS): a breakthrough for the post-genome malaria research2014

    • Author(s)
      Tsuboi T
    • Organizer
      Seminar at the Department of Parasite and Insect Vectors, Institut Pasteur
    • Place of Presentation
      Paris, France
    • Year and Date
      2014-12-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Wheat germ cell-free protein synthesis system: an innovative technology for post-genome malaria vaccine candidate discovery2014

    • Author(s)
      Tsuboi T
    • Organizer
      Seminar at the Laboratory of Malaria and Vector Research, NIAID, NIH
    • Place of Presentation
      Rockville, USA
    • Year and Date
      2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Antibodies against a Plasmodium falciparum rhoptry neck protein PfRON12 inhibit merozoite invasion into erythrocytes2014

    • Author(s)
      Ito D, Takashima E, Hatano S, Yamasaki T, Hasegawa T, Miura K, Thongkukiatkul A, Long CA, Sattabongkot J, Torii M, Tsuboi T
    • Organizer
      The 63rd annual meeting of the American Society of Tropical Medicine and Hygiene
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of the novel transmission-blocking vaccine target expressing on the surface of male gametes2014

    • Author(s)
      Tachibana M, Torii M, Sudo M, Tsuboi T, Ishino T
    • Organizer
      The 63rd annual meeting of the American Society of Tropical Medicine and Hygiene
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Immunoscreening of the target proteins contributing to growth inhibitory activity of human IgG against Plasmodium falciparum2014

    • Author(s)
      Morita M, Takashima E, Kanoi BN, Miura K, Tsuboi T
    • Organizer
      The 63rd annual meeting of the American Society of Tropical Medicine and Hygiene
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rhoptry proteins are involved in sporozoite invasion of the salivary gland2014

    • Author(s)
      Nozaki M, Ishino T, Tokunaga N, Tsuboi T, Torii M
    • Organizer
      The 63rd annual meeting of the American Society of Tropical Medicine and Hygiene
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2014-11-02 – 2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Global Health Innovation through Partnership2014

    • Author(s)
      Tsuboi T
    • Organizer
      第55回日本熱帯医学会大会・第29回日本国際保健医療学会学術大会合同大会
    • Place of Presentation
      東京女子医科大学、東京都
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Characterization of Plasmodium falciparum MAS170 as novel malaria blood-stage vaccine candidate2014

    • Author(s)
      Sasaoka C, Sakamoto H, Ito D, Takeo S, Sattabongkot J, Takashima E, Tsuboi T
    • Organizer
      The 13th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      奈良春日野国際フォーラム、奈良市、奈良県
    • Year and Date
      2014-09-23 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Wheat germ cell-free protein synthesis system: an innovative technology for post-genome malaria vaccine candidate discovery2014

    • Author(s)
      坪井敬文
    • Organizer
      第157回日本獣医学会学術集会、日本獣医寄生虫学会国際シンポジウム
    • Place of Presentation
      北海道大学、札幌市、北海道
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Wheat germ cell-free protein synthesis system (WGCFS): a breakthrough for the post-genome vivax malaria research2014

    • Author(s)
      Tsuboi T, Takashima E, Ito D, Feng Lu, Cheng Y, Han ET
    • Organizer
      13th International Congress of Parasitology
    • Place of Presentation
      Mexico City, Mexico
    • Year and Date
      2014-08-10 – 2014-08-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] 愛媛大学プロテオサイエンスセンターマラリア研究部門

    • URL

      http://www.pros.ehime-u.ac.jp/malaria/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター マラリア研究部門

    • URL

      http://www.pros.ehime-u.ac.jp/malaria/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report

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Published: 2014-11-20   Modified: 2022-02-07  

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