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Establishment of Molecular Imaging and Lipidomics Technologies for Early Diagnosis of Atherosclerotic Disease

Research Project

Project/Area Number 26253036
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOkayama University

Principal Investigator

MATSUURA Eiji  岡山大学, 医歯薬学総合研究科, 教授 (20181688)

Co-Investigator(Kenkyū-buntansha) 保田 晋助  北海道大学, 医学(系)研究科(研究院), 講師 (00374231)
Co-Investigator(Renkei-kenkyūsha) SASAKI Takanori  岡山大学, 医歯(薬)学総合研究科, 助教 (10461253)
KOBAYASHI Kazuko  岡山大学, 医歯(薬)学総合研究科, 助教 (20304298)
TAKENAKA Fumiaki  岡山大学, 医歯(薬)学総合研究科, 助教 (10642522)
OZEKI Eiichi  (株)島津製作, 基盤技術研究所, 研究員 (30192529)
FUJIWAKE Hideshi  (株)島津製作所, 分析計測事業部, 研究者 (50395696)
KOBUCHI Hirotsugu  岡山大学, 医歯(薬)学総合研究科, 講師 (10304297)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥41,860,000 (Direct Cost: ¥32,200,000、Indirect Cost: ¥9,660,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2014: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
Keywords動脈硬化 / Beta2-glycoprotein I / 血栓・止血 / 血管新生 / 抗リン脂質抗体症候群 / メタボロミクス / リピドミクス / LC-MS/MS / β2-グリコプロテインI (β2GPI) / 変異β2GPI-DV / 質量顕微鏡 / 酸化資質 / 酸化リポタンパク質 / LC-MSMS / PETイメージング / 分子イメージング / 脂質メタボローム / 標的医療 / 質量分析 / β2-グリコプロテインI (β2GPI) / 変異 β2GPI-DV / 酸化脂質 / PET イメージング / 酸化低密度リポタンパク質 (oxLDL)
Outline of Final Research Achievements

B2-Glycoprotein I (B2GPI) is a multifunctional plasma protein consisting of five homologous domains that control thrombosis/hemostasis and angiogenesis. Its physiological activity and binding affinity to particular phospholipids, oxidized lipids and/or proteins are regulated by the enzymatic activity of plasmin. In the present study, we identified a novel cleavage site by plasmin and produced plasmin-resistant recombinant proteins of domain V and domain I. With these, we have confirmed their localization at angiogenic legions in mouse xenograft models via PET imaging, and their respective physiological functions in vitro and in vivo. In addition, a metabolomic-based approach involving the combination of B2GPI-affinity column and LC-MS/MS has also been constructed to comprehensively analyse oxidized lipids involved in the development of atherosclerotic diseases.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (2 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] The Function of β2-glycoprotein I in Angiogenesis and Its in Vivo Distribution in Tumor Xenografts2016

    • Author(s)
      Arum Tri Wahyuningsih, Shen L, Kobayashi K, Sasaki T, Takenaka F, Hanada T, Akehi M, Akahoshi A, Ozeki E, Ando E, Matsuura E.
    • Journal Title

      Acta Medica Okayama

      Volume: 70(1) Pages: 13-24

    • NAID

      120005712601

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Harnessing Autoimmunity (Disease-Specific Autoantibody and Its Variant) in Theranostics2017

    • Author(s)
      Eiji Matsuura
    • Organizer
      he joined Conference of Lupus 2017 and Asian Autoimmunity Conference
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
    • Invited

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Published: 2014-04-04   Modified: 2018-03-22  

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