久保庭 雅恵 大阪大学, 歯学研究科, 准教授 (00303983)
竹内 洋輝 大阪大学, 歯学研究科(研究院), 助教 (40572186)
小島 美樹 大阪大学, 歯学研究科(研究院), 助教 (20263303)
橋野 恵衣 大阪大学, 歯学部附属病院, 特任助教 (90614553)
|Budget Amount *help
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2014: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
|Outline of Final Research Achievements
We analyzed the molecular basis involved in oral colonization of P. gingivalis. Coculture with P. gingivalis induced the secretions of arginine, agmatine and ornithine by F. alocis, which contributes to nutritional symbiosis of P. gingivalis. While, the pathogen is well known to invade host cells such as gingival epithelial cells. We showed that P. gingivalis was first internalized with early endosomes positive for Rab14 and RalA which are members of low molecular weitht GTP-binding proteins. Then, the intercellular pathogen was suggested to exploit these effector molecules for its intracellular inhabiting and exit from infected cells.
These findings suggest the molecular basis utilized by P. gingivalis for oral colonization.